1887

Abstract

A bacterium previously isolated from a diseased colony of the scleractinian coral (common name elliptical star coral) was subjected to a detailed polyphasic taxonomic characterization. The isolate, designated WP1, was halophilic and strictly aerobic and formed golden-orange-pigmented colonies after prolonged incubation. Cells of WP1 were Gram-negative, rod-shaped and showed a characteristic branching rod morphology. Chemotaxonomically, WP1 was characterized by having Q-10 as the major respiratory lipoquinone and -homospermidine as the main component of the cellular polyamine content. The predominant constituent in the cellular fatty acid profile was C 7, along with C cyclo 8 and C. Other fatty acids present in smaller amounts were C, C, C 7, C 7 and C 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Minor amounts of diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidyldimethylethanolamine were present. The G+C content of the genomic DNA was 66·3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that WP1 represents a separate subline of descent within the order ‘’ of the ‘’. The new line of descent falls within the group of families that includes the , , and ‘’, with no particular relative within this group. The 16S rRNA gene sequence similarity to all established taxa within this group was not higher than 92·0 % (to ). To accommodate this emerging coral pathogen, the creation of a new genus and species is proposed, gen. nov., sp. nov. (type strain WP1=CIP 107386 =DSM 14790).

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2003-07-01
2019-10-20
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