1887

Abstract

A novel chemolithotrophic bacterium, strain 16C, was isolated from a hot spring in Graendalur, south-west Iceland. Cells of this organism were Gram-negative, rod-shaped and motile. The isolate was aerobic and capable of chemolithotrophic growth on hydrogen and carbon dioxide, heterotrophic growth on butyrate and several other organic compounds, and mixotrophic growth on butyrate, hydrogen and carbon dioxide. Heterotrophic growth was generally enhanced in the presence of yeast extract. Autotrophic growth on hydrogen was observed at pH values between 6.0 and 10.0 and temperatures between 35 and 60 °C; optimum growth conditions were pH 7.0 and 55 °C. The DNA G+C content was 63.9 mol%. 16S rRNA gene sequence analysis showed that strain 16C was a member of a distinct species belonging to the class and was most closely related to NBRC 14978 and DSM 11420. The major cellular fatty acids were straight-chain C (44.98 %) and C 7 (17.93 %), as well as cyclic C (13.90 %) and C 8 (4.67 %) fatty acids. Based on its physiological and molecular properties, it is concluded that strain 16C represents a novel species within the genus , for which the name is proposed; the type strain is 16C (=DSM 21442=JCM 16106).

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2011-02-01
2024-04-25
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References

  1. Aragno M., Schlegel H. G. 1992; The mesophilic hydrogen-oxidizing (Knallgas) bacteria. In The Prokaryotes, 2nd edn. pp 344–384 Edited by Balows A., Trüper H., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  2. DSMZ 2006; Medium 81: Mineral Medium for Chemolithotrophic Growth (H-3) . Retrieved October 10, 2006, from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf
  3. Goto E., Kodama T., Minoda Y. 1977; Isolation and culture conditions of thermophilic hydrogen bacteria. Agric Biol Chem 41:685–690 [CrossRef]
    [Google Scholar]
  4. Goto E., Kodama T., Minoda Y. 1978; Growth and taxonomy of thermophilic hydrogen bacteria. Agric Biol Chem 42:1305–1308 [CrossRef]
    [Google Scholar]
  5. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  6. Hayashi N. R., Ishida T., Yokota A., Kodama T., Igarashi Y. 1999; Hydrogenophilus thermoluteolus gen. nov., sp. nov. a thermophilic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium. Int J Syst Bacteriol 49:783–786 [CrossRef]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  9. Miyake D., Ichiki S., Tanabe M., Oda T., Kuroda H., Nishihara H., Sambongi Y. 2007; Thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, Hydrogenophilus thermoluteolus . Arch Microbiol 188:199–204 [CrossRef]
    [Google Scholar]
  10. Orlygsson J., Baldursson S. R. 2007; Phylogenetic and physiological studies of four hydrogen-producing thermoanaerobes from Icelandic geothermal areas. Icelandic Agric Sci 20:93–105
    [Google Scholar]
  11. Skirnisdottir S., Hreggvidsson G. O., Hjöleifsdottir S., Marteinsson V. T., Petursdottir S. K., Holst O., Kristjansson J. K. 2000; Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. Appl Environ Microbiol 66:2835–2841 [CrossRef]
    [Google Scholar]
  12. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  13. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  14. Stöhr R., Waberski A., Liesack W., Völker H., Wehmeyer U., Thomm M. 2001; Hydrogenophilus hirschii sp. nov., a novel thermophilic hydrogen-oxidizing β -proteobacterium isolated from Yellowstone National Park. Int J Syst Evol Microbiol 51:481–488
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  16. Van de Peer Y., De Wachter R. 1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230
    [Google Scholar]
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