1887

Abstract

A novel chemolithotrophic bacterium, strain 16C, was isolated from a hot spring in Graendalur, south-west Iceland. Cells of this organism were Gram-negative, rod-shaped and motile. The isolate was aerobic and capable of chemolithotrophic growth on hydrogen and carbon dioxide, heterotrophic growth on butyrate and several other organic compounds, and mixotrophic growth on butyrate, hydrogen and carbon dioxide. Heterotrophic growth was generally enhanced in the presence of yeast extract. Autotrophic growth on hydrogen was observed at pH values between 6.0 and 10.0 and temperatures between 35 and 60 °C; optimum growth conditions were pH 7.0 and 55 °C. The DNA G+C content was 63.9 mol%. 16S rRNA gene sequence analysis showed that strain 16C was a member of a distinct species belonging to the class and was most closely related to NBRC 14978 and DSM 11420. The major cellular fatty acids were straight-chain C (44.98 %) and C 7 (17.93 %), as well as cyclic C (13.90 %) and C 8 (4.67 %) fatty acids. Based on its physiological and molecular properties, it is concluded that strain 16C represents a novel species within the genus , for which the name is proposed; the type strain is 16C (=DSM 21442=JCM 16106).

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2011-02-01
2019-10-14
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Analysis of fatty acids (%) of strain 16C and DSM 11420 . [ PDF] 33 KB

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Scanning electron micrographs of cells of strain 16C .

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