Three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [Phragmitesaustralis (Cav.) Trin. et Steudel] in Lake Fertő (Hungary). Cells of the novel isolates were Gram-negative, motile rods and formed star-shaped aggregates. They were facultatively anaerobic and chemo-organotrophic. Bacteriochlorophyll a was not synthesized under aerobic conditions. The strains were catalase and oxidase positive, produced acid from d-glucose under aerobic and anaerobic conditions and reduced nitrate to nitrogen. They tolerated pH values from 7·0 to 11·0 and grew in the absence of NaCl as well as in up to 5 % (w/v) NaCl. The G+C content of the DNA was 64·6 mol% and the major isoprenoid quinone was Q-10. The dominant cellular fatty acid was C18 : 1ω7c. The cell membrane contained phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl serine and one unknown phospholipid as polar lipids. Polyphasic taxonomic characterization revealed that strain C6/19T is most closely related to the Stappia–Roseibium cluster in the α-subclass of the Proteobacteria (showing 95·8–93·6 % 16S rDNA sequence similarity). According to the phylogenetic and phenotypic evidence presented, a new genus and species is proposed, Pannonibacter phragmitetus gen. nov., sp. nov. The type strain is C6/19T (=DSM 14782T=NCAIM B02025T).
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402[CrossRef]
BoettcherK. J.,
BarberB. J.,
SingerJ. T.2000; Additional evidence that juvenile oyster disease is caused by a member of the Roseobacter group and colonization of nonaffected animals by Stappia stellulata -like strains. Appl Environ Microbiol 66:3924–3930[CrossRef]
BorsodiA. K.,
FarkasI.,
KurdiP.1998; Numerical analysis of planktonic and reed biofilm bacterial communities of Lake Fertő (Neusiedlersee, Hungary/Austria. Water Res 32:1831–1840[CrossRef]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[CrossRef]
EscaraJ. F.,
HuttonJ. R.1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327[CrossRef]
GrothI.,
SchumannP.,
WeissN.,
MartinK.,
RaineyF. A.1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239[CrossRef]
GrothI.,
SchumannP.,
RaineyF. A.,
MartinK.,
SchuetzeB.,
AugstenK.1997; Demetria terragena gen. nov., sp. nov. a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133[CrossRef]
HughR.,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by Gram negative bacteria. J Bacteriol 66:24–26
HußV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
JahnkeK. D.1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Bacteriol 47:87–95[CrossRef]
MurrayR. G. E.,
DoetschR. N.,
RobinowC. F.1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41Edited byGerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
RaineyF. A.,
Ward-RaineyN.,
KroppenstedtR. M.,
StackebrandtE.1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 603–711Edited byGerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
SteadD. E.,
SellwoodJ. E.,
WilsonJ.,
VineyI.1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72:315–321[CrossRef]
StrunkO.,
LudwigW.1995arb – a software environment for sequence data Department of Microbiology, Technical University of Munich; Germany: http://www.biol.chemie.tu-muenchen.de/pub/ARB/
SuzukiT.,
MurogaY.,
TakahamaM.,
NishimuraY.2000; Roseibium denhamense gen. nov., sp. nov. and Roseibium hamelinense sp. nov. aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of Australia. Int J Syst Evol Microbiol 50:2151–2156[CrossRef]
YamadaK.,
KomagataK.1970; Taxonomic studies on coryneform bacteria. II. Principal amino acids in the cell wall and their taxonomic significance. J Gen Appl Microbiol 16:103–113[CrossRef]
YamadaK.,
KomagataK.1972; Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical and physiological characteristics. J Gen Appl Microbiol 18:399–416[CrossRef]