1887

Abstract

Three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [ (Cav.) Trin. Steudel] in Lake Fertő (Hungary). Cells of the novel isolates were Gram-negative, motile rods and formed star-shaped aggregates. They were facultatively anaerobic and chemo-organotrophic. Bacteriochlorophyll was not synthesized under aerobic conditions. The strains were catalase and oxidase positive, produced acid from -glucose under aerobic and anaerobic conditions and reduced nitrate to nitrogen. They tolerated pH values from 7·0 to 11·0 and grew in the absence of NaCl as well as in up to 5 % (w/v) NaCl. The G+C content of the DNA was 64·6 mol% and the major isoprenoid quinone was Q-10. The dominant cellular fatty acid was C 7. The cell membrane contained phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl serine and one unknown phospholipid as polar lipids. Polyphasic taxonomic characterization revealed that strain C6/19 is most closely related to the cluster in the -subclass of the (showing 95·8–93·6 % 16S rDNA sequence similarity). According to the phylogenetic and phenotypic evidence presented, a new genus and species is proposed, gen. nov., sp. nov. The type strain is C6/19 (=DSM 14782=NCAIM B02025).

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2003-03-01
2019-10-22
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vol. , part 2, pp. 555 - 561

Details of API 50CH and Biolog GN2 results for isolates of gen. nov., sp. nov. [PDF](16 KB)



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