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Abstract

A novel actinomycete, strain KZ0017, was isolated from a forest soil collected in Ohnuma, Fukushima, Japan. Strain KZ0017 formed spore chains borne on top of short sporophores arising from vegetative hyphae. Spores were non-motile and cylindrical with smooth surfaces. Strain KZ0017 contained -diaminopimelic (Apm) acid, 3-OH Apm, -glutamic acid, glycine and -alanine in the cell-wall peptidoglycan, and xylose, mannose, galactose, rhamnose and ribose in cell-wall hydrolysates. The acyl type of the cell-wall polysaccharides was glycolyl. The predominant menaquinones were MK-10(H) and MK-10(H); MK-10(H) was a minor component. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and several unknown lipids and glycolipids. The major fatty acids were iso-C, 10-methyl-C and iso-Cω9. The DNA G+C content was 70.7 mol%. The 16S rRNA gene sequence of the isolate formed a monophyletic cluster with the single member of the genus in the family . On the basis of morphological, chemotaxonomic and phylogenetic properties, strain KZ0017 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is KZ0017 ( = NBRC 105670 = DSM 45356).

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2011-04-01
2019-12-08
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Scanning electron micrographs of spore chains (a) and a bundle-like structure of aerial hyphae (b) of strain KZ0017 . Strain KZ0017 was grown on ISP 2 medium for 3 weeks (a) and on ISP 4 medium for 4 weeks (b) at 28°C. Bars, 1.0µm (a); 2.5µm (b).

IMAGE

Scanning electron micrographs of spore chains (a) and a bundle-like structure of aerial hyphae (b) of strain KZ0017 . Strain KZ0017 was grown on ISP 2 medium for 3 weeks (a) and on ISP 4 medium for 4 weeks (b) at 28°C. Bars, 1.0µm (a); 2.5µm (b).

IMAGE

Scanning electron micrographs of spore chains (a) and a bundle-like structure of aerial hyphae (b) of strain KZ0017 . Strain KZ0017 was grown on ISP 2 medium for 3 weeks (a) and on ISP 4 medium for 4 weeks (b) at 28°C. Bars, 1.0µm (a); 2.5µm (b).

IMAGE

Cellular fatty acid contents (%) of strain KZ0017 and K97-0003 . [ PDF] 39 KB

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