1887

Abstract

A novel actinomycete, strain KZ0017, was isolated from a forest soil collected in Ohnuma, Fukushima, Japan. Strain KZ0017 formed spore chains borne on top of short sporophores arising from vegetative hyphae. Spores were non-motile and cylindrical with smooth surfaces. Strain KZ0017 contained -diaminopimelic (Apm) acid, 3-OH Apm, -glutamic acid, glycine and -alanine in the cell-wall peptidoglycan, and xylose, mannose, galactose, rhamnose and ribose in cell-wall hydrolysates. The acyl type of the cell-wall polysaccharides was glycolyl. The predominant menaquinones were MK-10(H) and MK-10(H); MK-10(H) was a minor component. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and several unknown lipids and glycolipids. The major fatty acids were iso-C, 10-methyl-C and iso-Cω9. The DNA G+C content was 70.7 mol%. The 16S rRNA gene sequence of the isolate formed a monophyletic cluster with the single member of the genus in the family . On the basis of morphological, chemotaxonomic and phylogenetic properties, strain KZ0017 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is KZ0017 ( = NBRC 105670 = DSM 45356).

Funding
This study was supported by the:
  • High-tech Research Center Project of the Ministry of Education, Culture, Sports, Science and Technology, Japan (Award 2009-2011)
  • Institute for Fermentation, Osaka, Japan
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023531-0
2011-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/804.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023531-0&mimeType=html&fmt=ahah

References

  1. Bartholomew J. W., Mittwer T. 1952; The Gram stain. Bacteriol Rev 16:1–29[PubMed]
    [Google Scholar]
  2. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243[PubMed]
    [Google Scholar]
  3. Chiba H., Inokoshi J., Okamoto M., Asanuma S., Matsuzaki K., Iwama M., Mizumoto K., Tanaka H., Oheda M. et al. 2001; Actinohivin, a novel anti-HIV protein from an actinomycete that inhibits syncytium formation: isolation, characterization, and biological activities. Biochem Biophys Res Commun 282:595–601 [View Article][PubMed]
    [Google Scholar]
  4. Chiba H., Inokoshi J., Nakashima H., Ōmura S., Tanaka H. 2004; Actinohivin, a novel anti-human immunodeficiency virus protein from an actinomycete, inhibits viral entry to cells by binding high-mannose type sugar chains of gp120. Biochem Biophys Res Commun 316:203–210 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  7. Galtier N., Gouy M., Gautier C. 1996; SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548[PubMed]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coelica, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  9. Hamada M. 2001; Cultural, physiological and biochemical characteristics in actinomycetes. In Identification Manual of Actinomycetes, 1st edn. Chapter 2 pp. 37–47 Edited by Miyadoh S. , Hamada M., Hotta K., Kudo T., Seino A., Suzuki K., Yokota A. Tokyo: Business Center for Academic Societies Japan;
    [Google Scholar]
  10. Harper J. J., Davis G. H. G. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int J Syst Bacteriol 29:56–58 [View Article]
    [Google Scholar]
  11. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
    [Google Scholar]
  12. Hayakawa M., Nonomura H. 1989; A new method for the intensive isolation of actinomycetes from soil. Actinomycetologica 3:95–104 [View Article]
    [Google Scholar]
  13. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985; Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces. A Laboratory Manual pp. 72–74 Edited by Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. Norwich: John Innes Foundation;
    [Google Scholar]
  14. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146:527–534[PubMed]
    [Google Scholar]
  15. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  17. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  18. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  19. Matsumoto A., Takahashi Y., Shinose M., Seino A., Iwai Y., Ōmura S. 2003; Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae. . Int J Syst Evol Microbiol 53:1553–1559 [View Article][PubMed]
    [Google Scholar]
  20. Munsell Color Co. Inc. 1976 Munsell Book of Color – Matte Finish Collection Baltimore: Munsell Color Co., Inc;
    [Google Scholar]
  21. Nishijima M., Araki-Sakai M., Sano H. 1997; Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [View Article]
    [Google Scholar]
  22. Rayner R. W. (1970). A Mycological Colour Chart. Kew, UK: Commonwealth Mycological Institute & British Mycological Society.
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S ribosomal-RNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  27. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  28. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  29. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151:828–837[PubMed]
    [Google Scholar]
  30. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol 23:249–260 [View Article]
    [Google Scholar]
  31. Ueda K., Tagami Y., Kamihara Y., Shiratori H., Takano H., Beppu T. 2008; Isolation of bacteria whose growth is dependent on high levels of CO2 and implications of their potential diversity. Appl Environ Microbiol 74:4535–4538 [View Article][PubMed]
    [Google Scholar]
  32. Wang X., Hoefel D., Saint C. P., Monis P. T., Jin B. 2007; The isolation and microbial community analysis of hydrogen producing bacteria from activated sludge. J Appl Microbiol 103:1415–1423 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023531-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023531-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error