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Abstract

Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35, G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C, C, sometimes C, C and C in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the / group, with as their closest relative (about 95 % sequence similarity). DNA–DNA hybridization data indicated that the thermotolerant strains were not . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus , for which the name is proposed (G 35 =DSM 14792 =JCM 11425).

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2003-01-01
2019-10-14
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vol. , part 1, pp. 263-268

A fuller phylogenetic tree showing the placement of within the genus and a growth curve for strain G 35T are available as supplementary data when you click on the link below.



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