1887

Abstract

A non-motile, rod-shaped, red-pigmented bacterium, designated strain BZ33r, was isolated from soil of an industrial site. Cells stained Gram-negative and were aerobic and psychrophilic, showing good growth at 1–20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ33r was related to members of the genus and had highest sequence similarity with DSM 13606 (97.5 %). The predominant cellular fatty acids were anteiso-C (20.3 %), summed feature 3 (Cω7 and/or iso-C 2-OH; 20.2 %), iso-C (20.0 %), summed feature 4 (iso-C I and/or anteiso-C B; 8.2 %) and Cω5 (7.9 %). Strain BZ33r had MK-7 as the major menaquinone. The polyamine pattern contained -homospermidine as the major compound with moderate amounts of spermidine. Phosphatidylethanolamine, three unknown aminophospholipids, two unknown aminolipids, two unknown glycolipids and five unknown polar lipids were present in the polar lipid profile. The G+C content of the DNA was 61.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain BZ33r is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BZ33r ( = DSM 22290  = CGMCC 1.8975  = LMG 25548).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023465-0
2011-04-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/859.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023465-0&mimeType=html&fmt=ahah

References

  1. Baik K. S. , Seong C. N. , Moon E. Y. , Park Y.-D. , Yi H. , Chun J. . ( 2006; ). Hymenobacter rigui sp. nov., isolated from wetland freshwater. . Int J Syst Evol Microbiol 56:, 2189–2192. [CrossRef] [PubMed]
    [Google Scholar]
  2. Buczolits S. , Denner E. B. M. , Vybiral D. , Wieser M. , Kämpfer P. , Busse H.-J. . ( 2002; ). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.. Int J Syst Evol Microbiol 52:, 445–456.[PubMed]
    [Google Scholar]
  3. Buczolits S. , Denner E. B. M. , Kämpfer P. , Busse H.-J. . ( 2006; ). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. . Int J Syst Evol Microbiol 56:, 2071–2078. [CrossRef] [PubMed]
    [Google Scholar]
  4. Busse H.-J. , Auling G. . ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11:, 1–8.[CrossRef]
    [Google Scholar]
  5. Busse H.-J. , Bunka S. , Hensel A. , Lubitz W. . ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  6. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  7. Collins M. D. , Hutson R. A. , Grant I. R. , Patterson M. F. . ( 2000; ). Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov.. Int J Syst Evol Microbiol 50:, 731–734.[PubMed] [CrossRef]
    [Google Scholar]
  8. Dai J. , Wang Y. , Zhang L. , Tang Y. , Luo X. , An H. , Fang C. . ( 2009; ). Hymenobacter tibetensis sp. nov., a UV-resistant bacterium isolated from Qinghai-Tibet plateau. . Syst Appl Microbiol 32:, 543–548. [CrossRef] [PubMed]
    [Google Scholar]
  9. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein, J. (2009). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Hirsch P. , Ludwig W. , Hethke C. , Sittig M. , Hoffmann B. , Gallikowski C. A. . ( 1998; ). Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. . Syst Appl Microbiol 21:, 374–383.[PubMed] [CrossRef]
    [Google Scholar]
  12. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  13. Kates M. . ( 1986; ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  14. Kim K.-H. , Im W.-T. , Lee S.-T. . ( 2008; ). Hymenobacter soli sp. nov., isolated from grass soil. . Int J Syst Evol Microbiol 58:, 941–945. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  16. Margesin R. , Gander S. , Zacke G. , Gounot A. M. , Schinner F. . ( 2003; ). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles 7:, 451–458. [CrossRef] [PubMed]
    [Google Scholar]
  17. Reasoner D. J. , Geldreich E. E. . ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  18. Sambrook J. , Frisch E. F. , Maniatis T. . ( 1989; ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  19. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI Inc.
  20. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  21. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  22. Stolz A. , Busse H.-J. , Kämpfer P. . ( 2007; ). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:, 572–576. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  25. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  26. Xu J.-L. , Liu Q.-M. , Yu H.-S. , Jin F.-X. , Lee S.-T. , Im W.-T. . ( 2009; ). Hymenobacter daecheongensis sp. nov., isolated from stream sediment. . Int J Syst Evol Microbiol 59:, 1183–1187. [CrossRef] [PubMed]
    [Google Scholar]
  27. Zhang D.-C. , Wang H.-X. , Liu H.-C. , Dong X.-Z. , Zhou P.-J. . ( 2006; ). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. . Int J Syst Evol Microbiol 56:, 2921–2925. [CrossRef] [PubMed]
    [Google Scholar]
  28. Zhang Q. , Liu C. , Tang Y. , Zhou G. , Shen P. , Fang C. , Yokota A. . ( 2007; ). Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 57:, 1752–1756. [CrossRef] [PubMed]
    [Google Scholar]
  29. Zhang G. , Niu F. , Busse H.-J. , Ma X. , Liu W. , Dong M. , Feng H. , An L. , Cheng G. . ( 2008; ). Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai–Tibet Plateau permafrost region. . Int J Syst Evol Microbiol 58:, 1215–1220. [CrossRef] [PubMed]
    [Google Scholar]
  30. Zhang L. , Dai J. , Tang Y. , Luo X. , Wang Y. , An H. , Fang C. , Zhang C. . ( 2009; ). Hymenobacter deserti sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 59:, 77–82. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023465-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023465-0
Loading

Data & Media loading...

Supplements

Two-dimensional thin-layer chromatography (Merck silica gel 60 F aluminium-backed thin-layer plates) of polar lipids of strain BZ33r . PE, Phosphatidylethanolamine; APL1–3, unknown aminophospholipids; AL1–2, unknown aminolipids; GL1–2, unknown glycolipids; L1–5, unknown polar lipids.

IMAGE

Cellular fatty acid compositions of sp. nov. BZ33r and type strains of other species. [PDF](52 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error