1887

Abstract

Two actinomycete strains, ID05-A0653 and ID06-A0464, were isolated from soils of West Timor and Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family and were closely related to the genus . Strains ID05-A0653 and ID06-A0464 exhibited 98.1 and 98.2 % 16S rRNA gene sequence similarity, respectively, with GMKU 367. The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained -diaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H) as the sole isoprenoid quinone, iso-C as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G+C contents of strains ID05-A0653 and ID06-A0464 were 69.7 and 71.2 mol%, respectively. On the basis of phenotypic characteristics, DNA–DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653 and ID06-A0464 each represent a novel species of the genus for which the names sp. nov. (type strain ID05-A0653  = BTCC B-673  = NBRC 105524) and sp. nov. (type strain ID06-A0464  = BTCC B-674  = NBRC 105525) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023432-0
2011-04-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/834.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023432-0&mimeType=html&fmt=ahah

References

  1. Embley T. M. , Simida J. , Stackebrandt E. . ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov.. Syst Appl Microbiol 11:, 44–52.[CrossRef]
    [Google Scholar]
  2. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  3. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  4. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  5. Hasegawa T. , Tanida S. , Hatano K. , Higashide E. , Yoneda M. . ( 1983; ). Motile actinomycetes: Actinosynnema pretiosum subsp. pretiosum sp. nov., subsp. nov., and Actinosynnema pretiosum subsp. auranticum subsp. nov.. Int J Syst Bacteriol 33:, 314–320. [CrossRef]
    [Google Scholar]
  6. Hatano K. , Nishii T. , Kasai H. . ( 2003; ). Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA–DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev.. Int J Syst Evol Microbiol 53:, 1519–1529. [CrossRef] [PubMed]
    [Google Scholar]
  7. Hayakawa M. , Nonomura H. . ( 1987; ). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  8. Hayakawa M. , Nonomura H. . ( 1989; ). A new method for the intensive isolation of actinomycetes from soil. . Actinomycetologica 3:, 95–104. [CrossRef]
    [Google Scholar]
  9. Hayakawa M. , Otoguro M. , Takeuchi T. , Yamazaki T. , Iimura Y. . ( 2000; ). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. . Antonie van Leeuwenhoek 78:, 171–185. [CrossRef] [PubMed]
    [Google Scholar]
  10. Henssen A. . ( 1957; ). [Morphology and systematics of thermophilic actinomycetes]. . Arch Mikrobiol 26:, 373–414 (in German). [CrossRef] [PubMed]
    [Google Scholar]
  11. Indananda C. , Matsumoto A. , Inahashi Y. , Takahashi Y. , Duangmal K. , Thamchaipenet A. . ( 2010; ). Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 60:, 1141–1146. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jiang Y. , Wiese J. , Tang S.-K. , Xu L.-H. , Imhoff J. F. , Jiang C.-L. . ( 2008; ). Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 58:, 408–413. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim S. B. , Goodfellow M. . ( 1999; ). Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov.. Int J Syst Bacteriol 49:, 507–512. [CrossRef] [PubMed]
    [Google Scholar]
  14. Korn-Wendisch F. , Rainey F. , Kroppenstedt R. M. , Kempf A. , Majazza A. , Kutzner H. J. , Stackebrandt E. . ( 1995; ). Thermocrispum gen. nov., a new genus of the order Actinomycetales, and description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.. Int J Syst Bacteriol 45:, 67–77. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kusunoki S. , Ezaki T. , Tamesada M. , Hatanaka Y. , Asano K. , Hashimoto Y. , Yabuuchi E. . ( 1991; ). Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species. . J Clin Microbiol 29:, 1596–1603.[PubMed]
    [Google Scholar]
  16. Labeda D. P. . ( 2001; ). Crossiella gen. nov., a new genus related to Streptoalloteichus . . Int J Syst Evol Microbiol 51:, 1575–1579.[PubMed] [CrossRef]
    [Google Scholar]
  17. Labeda D. P. , Kroppenstedt R. M. . ( 2006; ). Goodfellowia gen. nov., a new genus of the Pseudonocardineae related to Actinoalloteichus, containing Goodfellowia coeruleoviolacea gen. nov., comb. nov.. Int J Syst Evol Microbiol 56:, 1203–1207. [CrossRef] [PubMed]
    [Google Scholar]
  18. Labeda D. P. , Kroppenstedt R. M. . ( 2008; ). Proposal for the new genus Allokutzneria gen. nov. within the suborder Pseudonocardineae and transfer of Kibdelosporangium albatum Tomita et al. 1993 as Allokutzneria albata comb. nov.. Int J Syst Evol Microbiol 58:, 1472–1475. [CrossRef] [PubMed]
    [Google Scholar]
  19. Labeda D. P. , Kroppenstedt R. M. , Euzéby J. P. , Tindall B. J. . ( 2008; ). Proposal of Goodfellowiella gen. nov. to replace the illegitimate genus name Goodfellowia Labeda and Kroppenstedt 2006. . Int J Syst Evol Microbiol 58:, 1047–1048. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lacey J. , Goodfellow M. . ( 1975; ). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov.. J Gen Microbiol 88:, 75–85.[PubMed] [CrossRef]
    [Google Scholar]
  21. Lechevalier M. P. , De Bièvre C. , Lechevalier H. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  22. Lechevalier M. P. , Prauser H. , Labeda D. P. , Ruan J.-S. . ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36:, 29–37. [CrossRef]
    [Google Scholar]
  23. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  24. Nonomura H. , Ohara Y. . ( 1971; ). Distribution of actinomycetes in soil. . X. New genus and species of monosporic actinomycetes in soil. J Ferment Technol 49:, 895–903.
    [Google Scholar]
  25. Rayner R. W. . ( 1970; ). A Mycological Colour Chart. Kew, UK:: Commonwealth Mycological Institute and British Mycological Society;.
    [Google Scholar]
  26. Saito H. , Miura K. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  27. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Schaal K. P. . ( 1985; ). Identification of clinically significant actinomycetes and related bacteria using chemical techniques. . In Chemical Methods in Bacterial Systematics, pp. 359–381. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  29. Shearer M. C. , Colman P. M. , Ferrin R. M. , Nisbet L. J. , Nash C. H. . ( 1986; ). New genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.. Int J Syst Bacteriol 36:, 47–54. [CrossRef]
    [Google Scholar]
  30. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  31. Stackebrandt E. , Kroppenstedt R. M. , Jahnke K. D. , Kemmerling C. , Gürtler H. . ( 1994; ). Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . . Int J Syst Bacteriol 44:, 265–269. [CrossRef]
    [Google Scholar]
  32. Tamura T. , Hatano K. . ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:, 2119–2125.[PubMed] [CrossRef]
    [Google Scholar]
  33. Tamura T. , Nakagaito Y. , Nishii T. , Hasegawa T. , Stackebrandt E. , Yokota A. . ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:, 193–203. [CrossRef] [PubMed]
    [Google Scholar]
  34. Tamura T. , Liu Z. , Zhang Y. , Hatano K. . ( 2000; ). Actinoalloteichus cyanogriseus gen. nov., sp. nov.. Int J Syst Evol Microbiol 50:, 1435–1440.[PubMed] [CrossRef]
    [Google Scholar]
  35. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  36. Tian X.-P. , Zhi X.-Y. , Qiu Y.-Q. , Zhang Y.-Q. , Tang S.-K. , Xu L.-H. , Zhang S. , Li W.-J. . ( 2009; ). Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. . Int J Syst Evol Microbiol 59:, 222–228. [CrossRef] [PubMed]
    [Google Scholar]
  37. Tomita K. , Nakakita Y. , Hoshino Y. , Numata K. , Kawaguchi H. . ( 1987; ). New genus of the Actinomycetales: Streptoalloteichus hindustanus gen. nov., nom. rev.; sp. nov., nom. rev.. Int J Syst Bacteriol 37:, 211–213. [CrossRef]
    [Google Scholar]
  38. Wang Y. , Zhang Z. , Ruan J. . ( 1996; ). A proposal to transfer Microbispora bispora (Lechevalier 1965) to a new genus, Thermobispora gen. nov., as Thermobispora bispora comb. nov.. Int J Syst Bacteriol 46:, 933–938. [CrossRef] [PubMed]
    [Google Scholar]
  39. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  40. Zhi X.-Y. , Li W.-J. , Stackebrandt E. . ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59:, 589–608. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023432-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023432-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 834 - 838

Scanning electron micrographs of cells of sp. nov. ID05-A0653> (a, b) and sp. nov. ID06-A0464 > (c, d) after growth on HV agar at 28 °C for 2 weeks. Bars, 10 µm (a, d) and 5 µm (b, c).

Fatty acid contents of type strains of species of the genus

Strains: 1, sp. nov. ID05-A0653 ; 2, sp. nov. ID06-A0464 ; 3, NBRC 105245 . Values are percentages of total fatty acids and were determined in this study; –, <1% of total fatty acids.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error