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Abstract

A novel bacterial strain designated B3A, isolated from shrimp paste, was investigated by a polyphasic taxonomic approach. Cells stained Gram-positive and were aerobic, non-pigmented, sporulating and rod-shaped with a polar flagellum. 16S rRNA gene sequence analysis indicated that strain B3A belonged to the class and was a member of the family . Strain B3A shared low levels of 16S rRNA gene sequence similarity (<94.0 %) with members of other genera in the family and was most closely related to BH2 (93.8 % sequence similarity). The isolate was able to grow at 20–45 °C, with 0.5–15 % NaCl and at pH 6–9. Menaquinone with seven isoprene units (MK-7) was the major respiratory quinone and 16 : 0 iso, 15 : 0 anteiso and 14 : 0 iso were the major fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The characteristic diamino acid of the peptidoglycan was -diaminopimelic acid. The DNA G+C content was 45.3 mol%. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain B3A represents a novel genus and species in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is B3A ( = BCRC 17939  = LMG 24826).

Funding
This study was supported by the:
  • , National Science Council, Taiwan, ROC , (Award 96-2313-B-022-001-MY3 and NSC 96-2320-B-022-001-MY2)
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2011-05-01
2020-11-25
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