1887

Abstract

A novel bacterial strain designated B3A, isolated from shrimp paste, was investigated by a polyphasic taxonomic approach. Cells stained Gram-positive and were aerobic, non-pigmented, sporulating and rod-shaped with a polar flagellum. 16S rRNA gene sequence analysis indicated that strain B3A belonged to the class and was a member of the family . Strain B3A shared low levels of 16S rRNA gene sequence similarity (<94.0 %) with members of other genera in the family and was most closely related to BH2 (93.8 % sequence similarity). The isolate was able to grow at 20–45 °C, with 0.5–15 % NaCl and at pH 6–9. Menaquinone with seven isoprene units (MK-7) was the major respiratory quinone and 16 : 0 iso, 15 : 0 anteiso and 14 : 0 iso were the major fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The characteristic diamino acid of the peptidoglycan was -diaminopimelic acid. The DNA G+C content was 45.3 mol%. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain B3A represents a novel genus and species in the family for which the name gen. nov., sp. nov. is proposed. The type strain of is B3A ( = BCRC 17939  = LMG 24826).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023341-0
2011-05-01
2020-01-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/5/1023.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023341-0&mimeType=html&fmt=ahah

References

  1. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735.[PubMed] [CrossRef]
    [Google Scholar]
  2. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  3. Echigo A. , Fukushima T. , Mizuki T. , Kamekura M. , Usami R. . ( 2007; ). Halalkalibacillus halophilus gen. nov., sp. nov., a novel moderately halophilic and alkaliphilic bacterium isolated from a non-saline soil sample in Japan. . Int J Syst Evol Microbiol 57:, 1081–1085. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  7. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  8. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  9. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–12. [CrossRef]
    [Google Scholar]
  10. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  11. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the GC content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  12. Miller P. H. , Wiggs L. S. , Miller J. M. . ( 1995; ). Evaluation of AnaeroGen system for growth of anaerobic bacteria. . J Clin Microbiol 33:, 2388–2391.[PubMed]
    [Google Scholar]
  13. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal K. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  14. Murray P. R. , Baron E. J. , Pfaller M. A. , TeNover F. C. , Yolken R. H. . ( 1999; ). Manual of Clinical Microbiology, , 7th edn.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  15. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  16. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  19. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  20. Ventosa A. , Marquez M. C. , Kocur M. , Tindall B. J. . ( 1993; ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . . Int J Syst Bacteriol 43:, 245–248. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023341-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023341-0
Loading

Data & Media loading...

Supplements

Expanded phylogenetic tree based on 16S rRNA gene sequences of strain B3A and related taxa. Bar, 0.01 substitutions per nucleotide position.

IMAGE

Electron micrograph of cells of strain B3A . Bar, 3 µm.

IMAGE

Two-dimensional thin-layer chromatogram of the polar lipids of strain B3A (a) and BH2 (b). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PL, phospholipid; AL, aminolipid.

IMAGE

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error