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Abstract

Two Gram-negative, motile, aerobic bacterial strains, designated B2 and 1_C16_27, were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C cyclo), octadecanoic (C and C 3-OH), octadecenoic (C) and hexadecanoic (C) acids. The G+C content of the genomic DNA was 58.1–59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family . The phylogenetically closest relatives were (92.7–93.7 % 16S rRNA gene sequence similarity), (92.9–94.4 %) and (91.7–92.1 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2 and 1_C16_27 could be differentiated from each other and from members of the genera , and . Therefore, it is proposed that strains B2 and 1_C16_27 represent two novel species in a new genus, for which the names gen. nov., sp. nov. (the type species; type strain B2  = CGMCC 1.7692  = JCM 15775) and sp. nov. (type strain 1_C16_27  = CGMCC 1.10267  = JCM 16552  = CELMS EEUT 1C1627) are proposed.

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2011-08-01
2020-01-27
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Transmission electron micrographs of cells of strains B2 and 1_C16_27 . (a) Strain B2 , showing slightly ovoid rods with several polar flagella; (b) strain 1_C16_27 , showing short rods with a single flagellum; (c) ultrathin section of strain B2 , showing outer and cytoplasmic membrane of the cell; (d) ultrathin section of strain 1_C16_27 , showing outer and cytoplasmic membrane of the cell. Bars, 0.5 µm (a, c) and 0.2 µm (b, d).

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[PDF file of Supplementary Figures S2 and S3](70 KB)

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Two-dimensional TLC after staining with molybdophosphoric acid showing the total polar lipid profiles of strains B2 (a) and 1_C16_27 (b) and reference strains DSM 18995 (c), CGMCC 1.7002 (d) and DSM 19414 (e). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; GL, unidentified glycolipid; PL, unidentified phospholipid; L, unidentified lipid.

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Fatty acid compositions of the novel isolates and related type strains. [PDF](39 KB)

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