1887

Abstract

Two Gram-negative, motile, aerobic bacterial strains, designated B2 and 1_C16_27, were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C cyclo), octadecanoic (C and C 3-OH), octadecenoic (C) and hexadecanoic (C) acids. The G+C content of the genomic DNA was 58.1–59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family . The phylogenetically closest relatives were (92.7–93.7 % 16S rRNA gene sequence similarity), (92.9–94.4 %) and (91.7–92.1 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2 and 1_C16_27 could be differentiated from each other and from members of the genera , and . Therefore, it is proposed that strains B2 and 1_C16_27 represent two novel species in a new genus, for which the names gen. nov., sp. nov. (the type species; type strain B2  = CGMCC 1.7692  = JCM 15775) and sp. nov. (type strain 1_C16_27  = CGMCC 1.10267  = JCM 16552  = CELMS EEUT 1C1627) are proposed.

Funding
This study was supported by the:
  • Ministry of Science and Technology of China 863 Program (Award 2007AA021305)
  • National Natural Science Foundation of China (Award 40806066)
  • Zhejiang Provincial Natural Science Foundation of China (Award Y5080060)
  • Chinese Offshore Investigation and Assessment (Award 908-ZC-I-02)
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2011-08-01
2021-10-22
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References

  1. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  2. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology. Beijing: Scientific Press;
    [Google Scholar]
  3. Euzéby J. P. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 http://www.bacterio.cict.fr [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  6. Garrity G. M., Bell J. A., Lilburn T. 2005; Family VIII. Hyphomicrobiaceae Babudieri 1950, 589. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C p. 476 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  7. Gliesche C., Fesefeldt A., Hirsch P. 2005; Genus I. Hyphomicrobium Stutzer and Hartleb 1898, 76AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 476–494 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  8. Hwang C. Y., Cho B. C. 2008; Cucumibacter marinus gen. nov., sp. nov., a marine bacterium in the family Hyphomicrobiaceae . Int J Syst Evol Microbiol 58:1591–1597 [View Article][PubMed]
    [Google Scholar]
  9. Kates M. 1986 Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  12. Lee K.-B., Liu C.-T., Anzai Y., Kim H., Aono T., Oyaizu H. 2005; The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55:1907–1919 [View Article][PubMed]
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  15. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479:297–306 [View Article][PubMed]
    [Google Scholar]
  16. Mikhailov V. V., Romanenko L. A., Ivanova E. P. 2006; The genus Alteromonas and related proteobacteria. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol. 6 pp. 597–645 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  17. Nakagawa Y., Sakane T., Yokota A. 1996; Transfer of “Pseudomonas riboflavina” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev.. Int J Syst Bacteriol 46:16–22 [View Article][PubMed]
    [Google Scholar]
  18. Ryu S. H., Chung B. S., Le N. T., Jang H. H., Yun P. Y., Park W., Jeon C. O. 2008; Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea. Int J Syst Evol Microbiol 58:633–636 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Schlesner H. 2005; Genus X. Filomicrobium Schlesner 1988, 220VP (Effective publication: Schlesner 1987, 65). In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 518–520 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  24. Truu J., Kärme L., Talpsep E., Heinaru E., Vedler E., Heinaru A. 2003; Phytoremediation of solid oil shale waste from the chemical industry. Acta Biotechnol 23:301–307 [View Article]
    [Google Scholar]
  25. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. 2007; Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624 [View Article][PubMed]
    [Google Scholar]
  26. Xu X.-W., Wu Y.-H., Wang C.-S., Yang J.-Y., Oren A., Wu M. 2008; Marinobacter pelagius sp. nov., a moderately halophilic bacterium. Int J Syst Evol Microbiol 58:637–640 [View Article][PubMed]
    [Google Scholar]
  27. Xu H.-Y., Chen L.-P., Fu S.-Z., Fan H.-X., Zhou Y.-G., Liu S.-J., Liu Z.-P. 2009; Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater. Int J Syst Evol Microbiol 59:2297–2301 [View Article][PubMed]
    [Google Scholar]
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