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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 8

gen. nov., sp. nov. and sp. nov., novel members of the family Free

Abstract

Two Gram-negative, motile, aerobic bacterial strains, designated B2 and 1_C16_27, were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C cyclo), octadecanoic (C and C 3-OH), octadecenoic (C) and hexadecanoic (C) acids. The G+C content of the genomic DNA was 58.1–59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family . The phylogenetically closest relatives were (92.7–93.7 % 16S rRNA gene sequence similarity), (92.9–94.4 %) and (91.7–92.1 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2 and 1_C16_27 could be differentiated from each other and from members of the genera , and . Therefore, it is proposed that strains B2 and 1_C16_27 represent two novel species in a new genus, for which the names gen. nov., sp. nov. (the type species; type strain B2  = CGMCC 1.7692  = JCM 15775) and sp. nov. (type strain 1_C16_27  = CGMCC 1.10267  = JCM 16552  = CELMS EEUT 1C1627) are proposed.

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Funding
This study was supported by the:
  • Ministry of Science and Technology of China 863 Program (Award 2007AA021305)
  • National Natural Science Foundation of China (Award 40806066)
  • Zhejiang Provincial Natural Science Foundation of China (Award Y5080060)
  • Chinese Offshore Investigation and Assessment (Award 908-ZC-I-02)
© 2011 IUMS
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2011-08-01
2025-01-14
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Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae
Int J Syst Evol Microbiol 61, 1817 (2011); https://doi.org/10.1099/ijs.0.023325-0
/content/journal/ijsem/10.1099/ijs.0.023325-0
/content/journal/ijsem/10.1099/ijs.0.023325-0
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