1887

Abstract

Bacteria of the avian []–‘’ complex have been associated with different pathological conditions in birds, among which salpingitis and peritonitis in chickens of layer type seem to dominate. The aim of this study was to classify these bacteria by comparison of 37 strains tentatively classified as biovars of the avian []–‘’ complex or as . PFGE, AFLP and plasmid profiling showed that strains representing different biovars were genotypically different. Phylogenetic analysis of 22 strains characterized by 16S rRNA gene sequence comparison showed that strains classified as biovars 5, 8 and 9 were closely related to the suggested type strain of ‘’ (98·4–99·9 % similarity), whereas the remaining strains classified in 12 biovars or as were closely related to the type strain of (98·1–100 % similarity). The two groups were related at 95·7–97·1 % similarity. The closest similarity outside this group was 94·6 %, between biovar 15 and Bisgaard taxon 3. DNA–DNA hybridization was performed with 34 strains and showed binding above 85 % for strains of biovars 5 and 8, including the suggested type strain of ‘’. Two strains of (F 149 and F 279) were closely related at 79 % DNA binding to 27 strains of biovars 1, 3, 4, 11, 12, 17–20, 22 and 24. A new genus, gen. nov., is proposed to include the avian []–‘’– complex, since these taxa form a monophyletic unit with similarities above 95 % on the basis of 16S rRNA sequence comparison and they are unrelated to other genera of the family Pohl 1981. The new genus consists of Gram-negative, non-motile, rod-shaped or pleomorphic bacteria. The bacteria are catalase-, oxidase- and phosphatase-positive. Nitrate is reduced and acid is produced without gas formation from glycerol, (−)-ribose, (+)-xylose, (−)-mannitol, (−)-fructose, (+)-galactose, (+)-glucose, (+)-mannose, sucrose and raffinose. The genus can be separated from other genera of by differences in catalase, symbiotic growth, haemolysis, urease, indole, acid production from (+)-xylose, (−)-mannitol, (−)-sorbitol, (+)-mannose, maltose, raffinose and dextrin and ONPG and PNPG tests. Mutters . 1985 is transferred to the new genus as gen. nov., comb. nov. Genomospecies 1 of is proposed to include the former biovars 5 and 8 of the avian []–‘’ complex. The type strain of is F 149 (=ATCC 43329=NCTC 11413) and the reference strain of genomospecies 1 is CCM 5974.

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2003-01-01
2019-09-15
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