1887

Abstract

A Gram-positive, aerobic actinomycete, designated strain NEAU 119, was isolated from the rhizosphere of a fig tree and was characterized using a polyphasic approach. The isolate formed branching, non-fragmenting vegetative hyphae and produced black pigment on yeast extract/malt extract (ISP medium 2). The G+C content of the DNA was 76.6 mol%. The organism had chemotaxonomic characteristics typical of the genus and was closely related to the type strains of , and , currently the only three recognized species of the genus , sharing 16S rRNA gene similarities of 96.4, 96.6 and 98.1 %, respectively. However, the results of DNA–DNA hybridization studies demonstrated that the novel strain showed only 46.8 % relatedness with the type strain of . In addition, a set of phenotypic characteristics also readily distinguished strain NEAU 119 from the type strains of recognized species of the genus . According to the above data, it is proposed that strain NEAU 119 represents a novel species, sp. nov. The type strain of is NEAU 119 ( = CGMCC 4.5714 = NBRC 106685).

Funding
This study was supported by the:
  • , National Key Project for Basic Research , (Award 2010CB126102)
  • , National Natural Science Foundation of China , (Award 30771427 and 30571234)
  • , National Key Technology R&D Program , (Award 2006BAD31B)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023283-0
2011-05-01
2020-08-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/5/1165.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023283-0&mimeType=html&fmt=ahah

References

  1. Collins M. D. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–284 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Goodfellow M., Orchard V. A. 1974; Antibiotic sensitivity of some nocardioform bacteria and its value as a criterion for taxonomy. J Gen Microbiol 83:375–387[PubMed] [CrossRef]
    [Google Scholar]
  5. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  6. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  7. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  8. Itoh T., Kudo T., Seino A. 1987; Chemotaxonomic studies on new genera of actinomycetes proposed in Chinese papers. Actinomycetologica 1:43–59 [CrossRef]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  10. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, Special Publication 6 pp. 277–284 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  11. Lee Y. K., Kim H. W., Liu C. L., Lee H. K. 2003; A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245–250 [CrossRef][PubMed]
    [Google Scholar]
  12. Liu Z., Zhang Y., Yan X. 1984; A new genus of the order Actinomycetales . Acta Microbiol Sin 24:295–298
    [Google Scholar]
  13. Loqman S., Barka E. A., Clément C., Ouhdouch Y. 2009; Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25:81–91 [CrossRef]
    [Google Scholar]
  14. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [CrossRef]
    [Google Scholar]
  15. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  16. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Singla A. K., Mayilraj S., Kudo T., Krishnamurthi S., Prasad G. S., Vohra R. M. 2005; Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 55:2561–2564 [CrossRef][PubMed]
    [Google Scholar]
  20. Tamura T., Hatano K. 1998; Phylogenetic analyses on the strains belonging to invalidated genera of the order Actinomycetales . Actinomycetologica 12:15–28 [CrossRef]
    [Google Scholar]
  21. Tamura T., Zhiheng L., Yamei Z., Hatano K. 2000; Actinoalloteichus cyanogriseus gen. nov., sp. nov.. Int J Syst Evol Microbiol 50:1435–1440[PubMed] [CrossRef]
    [Google Scholar]
  22. Wang X., Yao J., Yu Z. 2005; GC-MS determination of fatty acids in arachidonic acid high-yield strain induced by low-energy ion implantation. Chem Pap 59:240–243
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  24. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
    [Google Scholar]
  25. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  26. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 43:805–812 [CrossRef]
    [Google Scholar]
  27. Zhang H. T., Zheng W., Huang J. Y., Luo H. L., Jin Y., Zhang W., Liu Z. H., Huang Y. 2006; Actinoalloteichus hymeniacidonis sp. nov., an actinomycete isolated from the marine sponge Hymeniacidon perleve . Int J Syst Evol Microbiol 56:2309–2312 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023283-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023283-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error