1887

Abstract

Actinomycete strain RB72 was isolated from woodland bluff soil in northern Alabama, USA, and shown to produce a broad spectrum bacteriocin. Based on morphological and chemotaxonomic characteristics, the strain was determined to belong to the genus . Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that it differed from those of the described streptomycetes available in public databases. The distinctive white aerial hyphae and lack of sporulation suggest a deficiency in the pathway of the organism. A combination of substrate utilization patterns, morphological and chemotaxonomic characteristics and DNA–DNA hybridization results supported the affiliation of strain RB72 to the genus and enabled the genotypic and phenotypic differentiation of strain RB72 from closely related reference strains. Strain RB72 therefore represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB72 ( = DSM 41917  = NRRL B-24574).

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2011-09-01
2019-10-21
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Whole-cell fatty acid composition of strain RB72 and related speciesof the genus [ PDF] (35 KB)

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Phase-contrast micrographs of strain RB72 . Growth of strain RB72 on nutrient medium with 0.4% glucose at 25 °C for 14 days shows extensively branching aerial hyphae with a lack of sporulation. Images (a) and (b) are ×400 magnification. Images (c), (d) and (e) are ×1000 magnification.

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Scanning electron micrographs of strain RB72 . Growth of strain RB72 on oatmeal medium at 25 °C for 14 days shows extensively branching aerial hyphae with a lack of sporulation. Bars, 6.0 µm.

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