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Abstract

A taxonomic study was carried out on a novel bacterial strain, designated B108, which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108 was observed in 1–15 % (w/v) NaCl and at 10–39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108 was most closely related to HJ50 (97.1 % sequence similarity), followed by 81-2 (96.6 %) and SMK-122 (95.2 %); other species shared <95.0 % sequence similarity. DNA–DNA hybridization tests showed that strain B108 had a low DNA–DNA relatedness to HJ50 and . 81-2 (48±4 % and 44±5 %, respectively). The predominant fatty acids were C, C 2-OH, summed feature 8 (Cω7/ω6) and Cω8 cyclo, which accounted for 84.2 % of the total cellular fatty acids. The G+C content of the chromosomal DNA was 63.6 mol%. The major respiratory quinone was ubiquinone 10 (Q10). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and some unidentified compounds were detected. These characteristics were in good agreement with those of members of the genus . The gene was also detected. According to its morphology, physiology, fatty acid composition and phylogenetic position based on 16S rRNA sequence data, the novel strain most appropriately belongs to the genus but can be readily distinguished from known species of this genus. Therefore, strain B108 represents a novel species, of the genus , for which the name sp. nov. is proposed. The type strain is B108 ( = 2PR52-14  = CCTCC AB 208233  = LMG 24622  = MCCC 1A01227).

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2011-09-01
2019-10-20
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Supplements

vol. , part 9, pp. 2040 - 2044

Maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic positions of strain B108 and representatives of related taxa.

Polar lipids of strain B108 .

Transmission electron micrograph of negatively stained cells of strain B108 grown on Marine Agar 2216 (BD) medium for 24 h at 28 °C.

Cellular fatty acid contents of strain B108T and related species of the genus

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