1887

Abstract

Thirty-one cellulolytic bacterial isolates from soils that were phenotypically very similar and phylogenetically highly related to strains were further characterized using a polyphasic taxonomic approach. By using repetitive extragenic palindromic DNA-PCR fingerprinting, six different fingerprints could be recognized among the isolates. Representative strains and four reference strains of the genus were used for DNA–DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70 % DNA binding. One group was formed by three isolates and LMG 2848 and a second group consisted of strains ACM 2601 and ACM 2603. Two isolates and LMG 2847 constituted single-member groups. For the remaining groups, three novel species are proposed: sp. nov. (six strains, type strain LMG 18561 =ACM 5172), sp. nov. (eight strains, type strain LMG 19434 =ACM 5173) and sp. nov. (12 strains, type strain LMG 18551 =ACM 5174). The novel species could be differentiated from each other and from , and on the basis of phenotypic features, their fatty acid compositions and the G+C content of their DNA.

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2003-03-01
2024-12-02
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References

  1. Blackall L. L., Hayward A. C., Sly L. I. 1985; Cellulolytic and dextranolytic gram-negative bacteria: revival of the genus Cellvibrio . J Appl Bacteriol 59:81–97 [CrossRef]
    [Google Scholar]
  2. Blackall L. L., Hayward A. C., Sly L. I. 1986; Cellvibrio nom. rev. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no: 20 Int J Syst Bacteriol 36:354–356 [CrossRef]
    [Google Scholar]
  3. Frisoni G., Baiardo M., Scandola M., Lednická D., Cnockaert M. C., Mergaert J., Swings J. 2001; Natural cellulose fibers: heterogeneous acetylation kinetics and biodegradation behavior. Biomacromolecules 2:476–482 [CrossRef]
    [Google Scholar]
  4. Humphry D. R., Black G. W., Cummings S. P. 2003; Reclassification of ‘ Pseudomonas fluorescens subsp. cellulosa ’ NCIMB 10462 (Ueda et al . 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. Int J Syst Evol Microbiol 53393–400 [CrossRef]
    [Google Scholar]
  5. Lednická D., Mergaert J., Cnockaert M. C., Swings J. 2000; Isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic fibres. Syst Appl Microbiol 23:292–299 [CrossRef]
    [Google Scholar]
  6. Logan N. A., Lebbe L., Hoste B. 7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  8. Mergaert J., Verdonck L., Kersters K., Swings J., Boeufgras J.-M., De Ley J. 1984; Numerical taxonomy of Erwinia species using API systems. J Gen Microbiol 130:1893–1910
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  11. Rademaker J. L. W., de Bruijn F. J. 1997; Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: Protocols, Applications and Overviews pp 151–171Edited by Caetano-Anollés G. , Gresshoff P. M. New York: Wiley;
    [Google Scholar]
  12. Rademaker J. L. W., Hoste B., Louws F. J., Kersters K., Swings J., Vauterin L., Vauterin P., de Bruijn F. J. 2000; Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50:665–677 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Skerman V. B. D., McGowan V., Sneath P. H. A. editors 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30225–420 [CrossRef]
    [Google Scholar]
  15. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
  20. Winogradsky S. 1929; Etudes sur la microbiologie du sol. Sur la dégradation de la cellulose dans le sol. Ann Inst Pasteur 43:529–633
    [Google Scholar]
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