1887

Abstract

Thirty-one cellulolytic bacterial isolates from soils that were phenotypically very similar and phylogenetically highly related to strains were further characterized using a polyphasic taxonomic approach. By using repetitive extragenic palindromic DNA-PCR fingerprinting, six different fingerprints could be recognized among the isolates. Representative strains and four reference strains of the genus were used for DNA–DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70 % DNA binding. One group was formed by three isolates and LMG 2848 and a second group consisted of strains ACM 2601 and ACM 2603. Two isolates and LMG 2847 constituted single-member groups. For the remaining groups, three novel species are proposed: sp. nov. (six strains, type strain LMG 18561 =ACM 5172), sp. nov. (eight strains, type strain LMG 19434 =ACM 5173) and sp. nov. (12 strains, type strain LMG 18551 =ACM 5174). The novel species could be differentiated from each other and from , and on the basis of phenotypic features, their fatty acid compositions and the G+C content of their DNA.

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2003-03-01
2019-10-18
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