1887

Abstract

A group of rhizobial strains isolated from nodules of multiple legume species grown in different geographical regions of China had identical 16S rRNA genes. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the novel strains formed a subclade in the genus together with , and , with 99.8 % gene sequence similarity between the strains. The DNA–DNA relatedness values between the representative strain CCBAU 05176 and ATCC 43677, S02 and CCBAU 01393 were 22.6 %, 8.9 % and 15.9 %, respectively. The novel strains were distinguished from recognized species of the genus by using a polyphasic approach, including PCR-based restriction fragment length polymorphism analysis (RFLP) of the 16S–23S intergenic spacer (IGS), phenotypic and physiological tests, sequence comparisons of housekeeping genes and cellular fatty acid profiles. Therefore, it is suggested that this group of strains represents a novel species for which the name sp. nov. is proposed. The type strain is CCBAU 05176 (=HAMBI 3039=LMG 25447).

Keyword(s): NJ, neighbour joining
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2011-03-01
2019-10-22
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vol. , part 3, pp. 580 - 586

Ribosomal IGS-RFLP patterns showing the genomic relationships between strains of sp. nov. and phylogenetically related reference strains. Phylogenetic tree constructed with gene sequences, showing the relationships of sp. nov. and closely related bacteria. Phylogenetic tree constructed using gene sequences showing the relationships of sp. nov. and closely related bacteria. Cellular fatty acid contents (%) of sp. nov. and type strains of closely related species of the genus . Combined file [ PDF] 257 KB



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