A Gram-negative, pleomorphic, aerobic, haloalkaliphilic archaeon, strain 167-74T, was isolated from commercial rock salt imported into Japan from China. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 167-74T is closely related to Halostagnicola larsenii XH-48T (98.3 %) and Halostagnicola kamekurae 194-10T (97.2 %). The major polar lipids of the isolate were C20C20 and C20C25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. A glycolipid was not detected, in contrast to the two existing, neutrophilic species of the genus Halostagnicola. The DNA G+C content of strain 167-74T was 60.7 mol%. and it gave DNA–DNA reassociation values of 19.5 and 18.8 %, respectively, with Hst. larsenii JCM 13463T and Hst. kamekurae 194-10T. Therefore, strain 167-74T represents a novel species, for which the name Halostagnicola alkaliphila sp. nov. is proposed, with the type strain 167-74T ( = JCM 16592T = CECT 7631T).
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