1887

Abstract

A Gram-negative, pleomorphic, aerobic, haloalkaliphilic archaeon, strain 167-74, was isolated from commercial rock salt imported into Japan from China. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 167-74 is closely related to XH-48 (98.3 %) and 194-10 (97.2 %). The major polar lipids of the isolate were CC and CC derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. A glycolipid was not detected, in contrast to the two existing, neutrophilic species of the genus . The DNA GC content of strain 167-74 was 60.7 mol%. and it gave DNA–DNA reassociation values of 19.5 and 18.8 %, respectively, with JCM 13463 and 194-10. Therefore, strain 167-74 represents a novel species, for which the name sp. nov. is proposed, with the type strain 167-74 ( = JCM 16592  = CECT 7631).

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2011-05-01
2019-12-05
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Phylogenetic tree derived from 16S rRNA gene sequences showing the position of strain 167-74 among related haloarchaea. [PDF](34 KB)

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TLC of polar lipids extracted from strain 167-74 and some other haloarchaea. Lanes: 1, JCM 8978 ; 2, JCM 13463 ; 3, 194-10 ; 4, strain 167-74 . PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; S-TGA, sulfated triglycosyl archaeol.

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