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Abstract
Two novel psychrophilic bacterial strains (ZF-6T and ZF-8T) were isolated from the China No. 1 glacier. Polyphasic taxonomy using physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belonged to the genus Flavobacterium, and that they were distinct from each other and also from the known species of this genus. Strains ZF-6T and ZF-8T are Gram-negative and both have an optimal growth temperature of 11 °C. Strain ZF-6T is able to grow at 0–20 °C, the G+C content of its genomic DNA is 34·4 mol% and the major fatty acids of ZF-6T are C16 : 1 ω7c (17·7 %) and C15 : 1 ω6c (12·7 %). Strain ZF-8T showed a strong ability to degrade organic macromolecules such as starch, CM-cellulose, pectin and chitin. Its DNA G+C content is 35·1 mol%, and the major fatty acids are C16 : 1 ω7c (18·2 %) and C15 : 0 (9·9 %). Phylogenetic analysis based on 16S rDNA sequences indicated that ZF-6T and ZF-8T belong to the genus Flavobacterium and represent two novel species. DNA–DNA hybridization also supported the status of the two new isolates. The names Flavobacterium xinjiangense sp. nov. (type strain, ZF-6T=AS 1.2749T=JCM 11314T) and Flavobacterium omnivorum sp. nov. (type strain, ZF-8T=AS 1.2747T=JCM 11313T) are proposed for the two new isolates.
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