1887

Abstract

Two novel psychrophilic bacterial strains (ZF-6 and ZF-8) were isolated from the China No. 1 glacier. Polyphasic taxonomy using physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belonged to the genus , and that they were distinct from each other and also from the known species of this genus. Strains ZF-6 and ZF-8 are Gram-negative and both have an optimal growth temperature of 11 °C. Strain ZF-6 is able to grow at 0–20 °C, the G+C content of its genomic DNA is 34·4 mol% and the major fatty acids of ZF-6 are C 7 (17·7 %) and C 6 (12·7 %). Strain ZF-8 showed a strong ability to degrade organic macromolecules such as starch, CM-cellulose, pectin and chitin. Its DNA G+C content is 35·1 mol%, and the major fatty acids are C 7 (18·2 %) and C (9·9 %). Phylogenetic analysis based on 16S rDNA sequences indicated that ZF-6 and ZF-8 belong to the genus and represent two novel species. DNA–DNA hybridization also supported the status of the two new isolates. The names sp. nov. (type strain, ZF-6=AS 1.2749=JCM 11314) and sp. nov. (type strain, ZF-8=AS 1.2747=JCM 11313) are proposed for the two new isolates.

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2003-05-01
2019-10-16
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