1887

Abstract

The taxonomic status of an actinobacterium that changed colour during growth, strain 42H12-1, isolated from deep-sea sediment collected from the Atlantic Ocean, was established using a combination of genotypic and phenotypic data. Strain 42H12-1 formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus . The highest sequence similarity by analysis was to CP153-2 (98.5 %) and the second-highest sequence similarity was to AA-826 (97.5 %). DNA–DNA relatedness of only 12 % ( 1.82 %) between strain 42H12-1 and . DSM 16678 differentiated them as members of separate genomic species. Colonies of strain 42H12-1 were black on oligotrophic medium, but orange to red, turning black, on copiotrophic medium. The peptidoglycan contained -diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unknown aminophospholipid. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C and Cω8. The DNA GC content was 72.3±1 mol%. Strain 42H12-1 ( = DSM 45201  = CGMCC 4.5581) is assigned as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2011-07-01
2020-01-21
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Maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic position of strain 42H12-1 . [PDF](41 KB)

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Colonies of DSM 16678 (left) and strain 42H12-1 (right) grown on ISP2 medium for 3 (a), 6 (b) or 15 (c) days. Colonies of the two strains are initially pink to deep orange. Melanin-like pigment production starts earlier and is greater in strain 42H12-1 and makes the colonies blacker than those of DSM 16678 .

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(a) Aminophospholipid of 42H12-1 detected by ninhydrin with phospholipid standard. (b, c) Comparison of phospholipids between DSM 16678 (b) and strain 42H12-1 (c). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PME, phosphatidylmethylethanolamine; PI, phosphatidylinositol; APL, unknown aminophospholipid; PE(s) and PME(s), standards.

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Transmission electron micrograph of a negatively stained cell of strain 42H12-1 . Flagella are visible. Bar, 1 µm.

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Comparison of fatty acid compositions of strain 42H12-1 and DSM 16678 . [PDF](35 KB)

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