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Abstract

The taxonomic status of an actinobacterium that changed colour during growth, strain 42H12-1, isolated from deep-sea sediment collected from the Atlantic Ocean, was established using a combination of genotypic and phenotypic data. Strain 42H12-1 formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus . The highest sequence similarity by analysis was to CP153-2 (98.5 %) and the second-highest sequence similarity was to AA-826 (97.5 %). DNA–DNA relatedness of only 12 % ( 1.82 %) between strain 42H12-1 and . DSM 16678 differentiated them as members of separate genomic species. Colonies of strain 42H12-1 were black on oligotrophic medium, but orange to red, turning black, on copiotrophic medium. The peptidoglycan contained -diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unknown aminophospholipid. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C and Cω8. The DNA GC content was 72.3±1 mol%. Strain 42H12-1 ( = DSM 45201  = CGMCC 4.5581) is assigned as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • , China Ocean Mineral Resources Research and Development Association fund , (Award DYXM-115-02-2-8)
  • , National High Technology R&D Program of China , (Award 2007AA091904)
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2011-07-01
2020-11-30
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