RT Journal Article SR Electronic(1) A1 Täubel, Martin A1 Kämpfer, Peter A1 Buczolits, Sandra A1 Lubitz, Werner A1 Busse, Hans-JürgenYR 2003 T1 Bacillus barbaricus sp. nov., isolated from an experimental wall painting JF International Journal of Systematic and Evolutionary Microbiology, VO 53 IS 3 SP 725 OP 730 DO https://doi.org/10.1099/ijs.0.02304-0 PB Microbiology Society, SN 1466-5034, AB In a project concerning bacterial colonization of experimental wall paintings, a large number of isolates have been acquired with high similarities in their whole-cell protein patterns obtained after SDS-PAGE. Of this group, four strains, designated V2-BIII-A2T, V2-BI-A9, V2-BI-04 and V2-BII-A8, were chosen for further characterization. Banding patterns obtained after ERIC-PCR were barely distinguishable among these four strains, indicating their affiliation within a single species. The isolates also displayed nearly identical biochemical and physiological features. The chemotaxonomic characteristics, including polar lipids, quinone systems, cell-wall diamino acid composition and fatty acid profiles, were in good agreement with those of numerous previously described Bacillus species. 16S rDNA analysis of strain V2-BIII-A2T showed that this bacterium belongs to the genus Bacillus, with highest sequence similarities to Bacillus megaterium (94·6 %), Bacillus flexus (94·4 %) and the alkaliphilic Bacillus cohnii (94·2 %). Based on almost identical biochemical, physiological and chemotaxonomic traits, ERIC-PCR-generated genomic fingerprints and comparative 16S rDNA sequence analysis, it is demonstrated that the four isolates represent a novel species of the genus Bacillus, for which the name Bacillus barbaricus sp. nov. is proposed. The type strain is V2-BIII-A2T (=CCM 4982T=DSM 14730T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02304-0