1887

Abstract

In a project concerning bacterial colonization of experimental wall paintings, a large number of isolates have been acquired with high similarities in their whole-cell protein patterns obtained after SDS-PAGE. Of this group, four strains, designated V2-BIII-A2, V2-BI-A9, V2-BI-04 and V2-BII-A8, were chosen for further characterization. Banding patterns obtained after ERIC-PCR were barely distinguishable among these four strains, indicating their affiliation within a single species. The isolates also displayed nearly identical biochemical and physiological features. The chemotaxonomic characteristics, including polar lipids, quinone systems, cell-wall diamino acid composition and fatty acid profiles, were in good agreement with those of numerous previously described species. 16S rDNA analysis of strain V2-BIII-A2 showed that this bacterium belongs to the genus , with highest sequence similarities to (94·6 %), (94·4 %) and the alkaliphilic (94·2 %). Based on almost identical biochemical, physiological and chemotaxonomic traits, ERIC-PCR-generated genomic fingerprints and comparative 16S rDNA sequence analysis, it is demonstrated that the four isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is V2-BIII-A2 (=CCM 4982=DSM 14730).

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2003-05-01
2021-03-07
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Schumann P., Vybiral D., Lubitz W., Busse H.-J. 2002; Georgenia muralis gen. nov., sp. nov. a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52:875–881 [CrossRef]
    [Google Scholar]
  3. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  4. Bousfield I. J., Keddie R. M., Dando T. R., Shaw S. 1985; Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. In Chemical Methods in Bacterial Systematics pp 221–236Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  5. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  6. Buczolits S., Denner E. B. M., Vybiral D., Wieser M., Kämpfer P., Busse H.-J. 2002; Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52:445–456
    [Google Scholar]
  7. Cifferi O. 1999; Microbial degradation of paintings. Appl Environ Microbiol 65:879–885
    [Google Scholar]
  8. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology vol. 2 pp 1105–1139Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Fox J. G., Dewhirst F. E., Tully J. G., Paster B. J., Yan L., Taylor N. S., Collins M. J. Jr, Gorelick P. L., Ward J. M. 1994; Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol 32:1238–1245
    [Google Scholar]
  10. Kämpfer P. 1994; Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17:86–98 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans ” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583 [CrossRef]
    [Google Scholar]
  13. Krumbein W. E., Petersen K. 1990; Mikroorganismen beschleunigen den Zerfall von mittelalterlichen Wandgemälden. Wandmalereischäden Arbeitshefte Denkmalplege Niedersachsen 8:115–121
    [Google Scholar]
  14. Moaledj K. 1986; Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy. J Microbiol Methods 5:303–310 [CrossRef]
    [Google Scholar]
  15. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  16. Petushkova Y. P., Lyalikova N. N., Poglasova M. N. 1989; Microorganisms discovered on the frescoes of the Ferapoint Monastery. Mikrobiologiya 58:1021–1030
    [Google Scholar]
  17. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
    [Google Scholar]
  18. Sorlini C., Sacchi M., Ferrari A. 1987; Microbiological deterioration of Gambara's frescos exposed to open air in Brescia, Italy. Int Biodeterior 23:167–179 [CrossRef]
    [Google Scholar]
  19. Spanka R., Fritze D. 1993; Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. Int J Syst Bacteriol 43:150–156 [CrossRef]
    [Google Scholar]
  20. Tindall B. J. 1990; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  21. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  22. Wieser M., Busse H.-J. 2000; Rapid identification of Staphylococcus epidermidis . Int J Syst Evol Microbiol 50:1087–1093 [CrossRef]
    [Google Scholar]
  23. Wieser M., Schumann P., Martin K., Altenburger P., Burghardt J., Lubitz W., Busse H.-J. 1999; Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49:1165–1170 [CrossRef]
    [Google Scholar]
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