1887

Abstract

In a project concerning bacterial colonization of experimental wall paintings, a large number of isolates have been acquired with high similarities in their whole-cell protein patterns obtained after SDS-PAGE. Of this group, four strains, designated V2-BIII-A2, V2-BI-A9, V2-BI-04 and V2-BII-A8, were chosen for further characterization. Banding patterns obtained after ERIC-PCR were barely distinguishable among these four strains, indicating their affiliation within a single species. The isolates also displayed nearly identical biochemical and physiological features. The chemotaxonomic characteristics, including polar lipids, quinone systems, cell-wall diamino acid composition and fatty acid profiles, were in good agreement with those of numerous previously described species. 16S rDNA analysis of strain V2-BIII-A2 showed that this bacterium belongs to the genus , with highest sequence similarities to (94·6 %), (94·4 %) and the alkaliphilic (94·2 %). Based on almost identical biochemical, physiological and chemotaxonomic traits, ERIC-PCR-generated genomic fingerprints and comparative 16S rDNA sequence analysis, it is demonstrated that the four isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is V2-BIII-A2 (=CCM 4982=DSM 14730).

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2003-05-01
2020-01-21
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