1887

Abstract

Slowly growing, non-pigmented mycobacteria were isolated from striped bass () during an epizootic of mycobacteriosis in the Chesapeake Bay. Growth characteristics, acid-fastness and results of 16S rRNA gene sequencing were consistent with those of the genus . A unique profile of biochemical reactions was observed among the 21 isolates. A single cluster of eight peaks identified by analysis of mycolic acids (HPLC) resembled those of reference patterns but differed in peak elution times from profiles of reference species of the complex. One isolate (M175) was placed within the slowly growing mycobacteria by analysis of aligned 16S rRNA gene sequences and was proximate in phylogeny to and . However, distinct nucleotide differences were detected in the 16S rRNA gene sequence among M175, . and . (99·2 % similarity). Isolate M175 could be differentiated from other slowly growing, non-pigmented mycobacteria by its inability to grow at 37 °C, production of niacin and urease, absence of nitrate reductase and resistance to isoniazid (1 µg ml), thiacetazone and thiophene-2-carboxylic hydrazide. Based upon these genetic and phenotypic differences, isolate M175 (=ATCC 700981 =NCTC 13215) is proposed as the type strain of a novel species, sp. nov.

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2003-03-01
2020-01-23
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