1887

Abstract

A taxonomic study was carried out on strain 10-D-4, which was isolated from a crude oil-degrading consortium enriched from surface seawater collected around Xiamen Island, PR China. Strain 10-D-4 grew optimally at pH 7.0–8.0 and at 25 °C. The 16S rRNA gene sequence of strain 10-D-4 showed the highest similarity to those of ISL-52 (94.6 %), PIN1 (94.2 %) and CL-SP19 (94.1 %), and showed lower similarity (92.3–94.0 %) to other members of the genus . The major isoprenoid quinone was ubiquinone 8 (Q-8). The major fatty acids were iso-C (5.2 %), iso-C (15.3 %), C (14.3 %), summed feature 3 (Cω6c and/or Cω7) (6.6 %), iso-C (15.4 %) and Cω7 (13.5 %). The G+C content of the chromosomal DNA was 50.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences, together with data from phenotypic and chemotaxonomic characterization, revealed that strain 10-D-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 10-D-4 ( = CCTCC AB 209061  = LMG 25227  = MCCC 1A01370). We also propose the transfer of , described recently, to the genus as comb. nov. (type strain KYW314  = KCTC 22740  = JCM 16344).

Funding
This study was supported by the:
  • , National Infrastructure of Natural Resources for Science and Technology Program of China , (Award 2005DKA21209)
  • , Scientific Research Foundation of the Third Institute of Oceanography, SOA , (Award 2009065)
  • , Fujian Science and Technology Foundation
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2011-04-01
2020-10-24
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  4. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov.. Int J Syst Evol Microbiol 50:901–907[PubMed] [CrossRef]
    [Google Scholar]
  5. Jean W. D., Shieh W. Y., Chiu H. H. 2006; Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56:899–905 [CrossRef][PubMed]
    [Google Scholar]
  6. Jean W. D., Leu T. Y., Lee C.-Y., Chu T. J., Lin S. Y., Shieh W. Y. 2009; Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. Int J Syst Evol Microbiol 59:53–59 [CrossRef][PubMed]
    [Google Scholar]
  7. Lai Q., Yuan J., Gu L., Shao Z. 2009; Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59:1278–1281 [CrossRef][PubMed]
    [Google Scholar]
  8. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef][PubMed]
    [Google Scholar]
  9. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479:297–306 [CrossRef][PubMed]
    [Google Scholar]
  10. Park S., Lim C., Baik K., Lee K., Lee J., Seong C. 2010; Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater. Int J Syst Evol Microbiol 60:2071–2075 [CrossRef]
    [Google Scholar]
  11. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  12. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095[PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sasser, M. (1997). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI.
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  16. Taborda M., Antunes A., Tiago I., Veríssimo A., Nobre M. F., da Costa M. S. 2009; Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . Syst Appl Microbiol 32:371–378 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  18. Wang L., Wang W., Lai Q., Shao Z. 2010; Gene diversity of CYP153A and AlkB alkane hydroxylases in oil-degrading bacteria isolated from the Atlantic Ocean. Environ Microbiol 12:1230–1242 [CrossRef][PubMed]
    [Google Scholar]
  19. Yoon J. H., Jung S. Y., Jung Y. T., Oh T. K. 2007; Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57:2503–2506 [CrossRef][PubMed]
    [Google Scholar]
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