1887

Abstract

Nine strains isolated from symbiotic root nodules on were previously designated as representing genospecies I. Phylogenetic analyses indicated that genospecies I was related closely to (genospecies II), and clearly formed a new lineage within the genus . In this study, we differentiated genospecies I from recognized species of the genus based on phylogenetic analyses of additional core genes (, ), levels of DNA–DNA relatedness (<43.3 %), fatty acid profile (58 % C 7, 19 % 11-methyl C 7), and biochemical and physiological characteristics. The nine strains are therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CCNWXJ 40-4 (=HAMBI 3020 =ACCC 14549).

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2011-03-01
2019-10-16
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References

  1. Chen, W.-M., Zhu, W.-F., Young, J. P. W., Bontemps, C. & Wei, G. H. ( 2010; ). Mesorhizobium alhagi sp. nov., isolated from wild Alhagi sparsifolia in north-western China. Int J Syst Evol Microbiol 60, 958–962.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  4. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lloyd-Macgilp, S. A. & Young, J. P. W. ( 2001; ). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef]
    [Google Scholar]
  5. Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O. ( 2005; ). phyml online–a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33 (Web Server), W557–W559.[CrossRef]
    [Google Scholar]
  6. Jarvis, B. D. W., van Berkum, P., Chen, W. X., Nour, S. M., Fernandez, M. P., Cleyet-Marel, J. C. & Gillis, M. ( 1997; ). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47, 895–898.[CrossRef]
    [Google Scholar]
  7. Laguerre, G., Nour, S. M., Macheret, V., Sanjuan, J., Drouin, P. & Amarger, N. ( 2001; ). Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147, 981–993.
    [Google Scholar]
  8. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  9. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  10. Siebert, S., Gries, D., Zhang, X., Runge, M. & Buerkert, A. ( 2004; ). Non-destructive dry matter estimation of Alhagi sparsifolia vegetation in a desert oasis of Northwest China. J Veg Sci 15, 365–372.
    [Google Scholar]
  11. Sneath, P. H. A. & Sokal, R. B. ( 1973; ). Numerical Taxonomy. The Principles and Practice of Numerical Classification. San Francisco. : W. H. Freeman.
    [Google Scholar]
  12. Sprent, J. I. ( 2001; ). Nodulation in Legumes. Kew. : Kew Publishing.
    [Google Scholar]
  13. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  14. Tighe, S. W., de Lajudie, P., Dipietro, K., Lindström, K., Nick, G. & Jarvis, B. D. W. ( 2000; ). Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50, 787–801.[CrossRef]
    [Google Scholar]
  15. Turner, S. L. & Young, J. P. W. ( 2000; ). The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol 17, 309–319.[CrossRef]
    [Google Scholar]
  16. Wei, G. H., Chen, W. M., Young, J. P. W. & Bontemps, C. ( 2009; ). A new clade of Mesorhizobium nodulating Alhagi sparsifolia. Syst Appl Microbiol 32, 8–16.[CrossRef]
    [Google Scholar]
  17. Zeng, F., Zhang, X., Foetzki, A., Li, X., Li, X. & Runge, M. ( 2002; ). Water relation characteristics of Alhagi sparsifolia and consequences for a sustainable management. Sci China Ser D: Earth Sci 45 (Suppl. 1), 125–131.[CrossRef]
    [Google Scholar]
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