1887

Abstract

A bacterial strain, designated SW6, was isolated from the reef-building coral , collected from seawater off the coast of southern Taiwan, and characterized using a polyphasic taxonomic approach. Strain SW6 was Gram-negative, aerobic, beige coloured, rod-shaped and motile by monopolar flagella. 16S rRNA gene sequence studies showed that the strain clustered closely with JCM 13038 (97.9 % 16S rRNA gene sequence similarity). Strain SW6 required NaCl for growth and exhibited optimal growth at 25–30 °C and 3–4 % NaCl. The predominant cellular fatty acid was summed feature 8 (Cω7/Cω6; 64.1 %). The major respiratory quinone was ubiquinone Q-10 and the DNA G+C content was 54.9 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from previously described species of the genus . It is evident from the genotypic, phenotypic and chemotaxonomic data that the new strain should be classified as a representative of a novel species in the genus . The name proposed for this taxon is sp. nov.; the type strain is SW6 ( = LMG 25377 = BCRC 80085).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022848-0
2011-04-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/823.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022848-0&mimeType=html&fmt=ahah

References

  1. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735.[PubMed] [CrossRef]
    [Google Scholar]
  2. Choi D. H. , Cho B. C. . ( 2006; ). Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. . Syst Appl Microbiol 56:, 1869–1873.
    [Google Scholar]
  3. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. GCG ( 1995; ). Wisconsin Package Version 8.1 Program Manual. Madison, WI:: Genetics Computer Group;.
    [Google Scholar]
  8. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  10. Hosoya S. , Adachi K. , Kasai H. . ( 2009; ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59:, 686–690. [CrossRef] [PubMed]
    [Google Scholar]
  11. Hsu S. C. , Lockwood J. L. . ( 1975; ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29:, 422–426.[PubMed]
    [Google Scholar]
  12. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  13. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  14. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lyman J. , Fleming R. H. . ( 1940; ). Composition of sea water. . J Mar Res 3:, 134–146.
    [Google Scholar]
  16. MacFaddin J. F. . ( 2000; ). Biochemical Tests for Identification of Medical Bacteria, , 3rd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  17. Maidak B. L. , Cole J. R. , Lilburn T. G. , Parker C. T. Jr , Saxman P. R. , Farris R. J. , Garrity G. M. , Olsen G. J. , Schmidt T. M. , Tiedje J. M. . ( 2001; ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal K. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  20. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022848-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022848-0
Loading

Data & Media loading...

vol. , part 4, pp. 823 - 827

GN2 microplate oxidation data useful for distinguishing strain SW6 from JCM 13038 . [ PDF] 37KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error