Two bacterial strains (KMM 3633T and KMM 3634) were isolated from marine coastal sea-ice and characterized by using phenotypic and molecular methods. The isolates were aerobic, Gram-negative, psychrophilic, halophilic and motile by means of a single polar flagellum. The DNA G+C content was 45·3–45·6 mol%. The major cellular fatty acids were C16 : 0, C16 : 1ω9c and C18 : 1ω7c. Comparison of almost-complete 16S rDNA sequences demonstrated that the strains were phylogenetically closely related to each other (99·5 % sequence identity), and related to Marinomonas species (94·4–96·4 % identity). DNA–DNA reassociation between KMM 3633T and KMM 3634 occurred at a level of 92 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, the name Marinomonas primoryensis sp. nov. is proposed for strains KMM 3633T and KMM 3634; the type strain is KMM 3633T (=JCM 11775T=NRIC 523T).
BaumannP., GauthierM. J., BaumannL.1984; Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972. In Bergey's Manual of Systematic Bacteriology vol 1 pp 343–352Edited byKriegN. R., HoltJ. G. Baltimore: Williams & Wilkins;
GauthierM. J., BreittmayerV. A.1992; The genera Alteromonas and Marinomonas . In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applicationsvol. 3 pp 3046–3070Edited byBalowsA., TruperH. G., DworkinM., HarderW., SchleiferK.-H. Berlin: Springer;
MarmurJ., DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
ReicheltJ. L., BaumannP.1973; Change of the name Alteromonas marinopraesens (ZoBell and Upham) Baumann et al. to Alteromonas haloplanktis (ZoBell and Upham) comb. nov. and assignment of strain ATCC 23821 ( Pseudomonas enalia) and strain c-A1 of De Voe and Oginsky to this species. Int J Syst Bacteriol 23:438–441[CrossRef]
ShidaO., TakagiH., KadowakiK., NakamuraL. K., KomagataK.1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298[CrossRef]
SmibertR. M., KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–655Edited byGerhardtP., MurrayR. G. E., WoodW. A., KriegN. R. Washington, DC: American Society for Microbiology;
SolanoF., GarcíaE., Pérez de EgeaE., Sanchez-AmatA.1997; Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl Environ Microbiol 63:3499–3506
SolanoF., Sanchez-AmatA.1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–1246[CrossRef]
StackebrandtE., GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
Van LandschootA., De LeyJ.1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
WayneL. G., BrennerD. J., ColwellR. R.9 other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]