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Two bacterial strains (KMM 3633T and KMM 3634) were isolated from marine coastal sea-ice and characterized by using phenotypic and molecular methods. The isolates were aerobic, Gram-negative, psychrophilic, halophilic and motile by means of a single polar flagellum. The DNA G+C content was 45·3–45·6 mol%. The major cellular fatty acids were C16 : 0, C16 : 1 ω9c and C18 : 1 ω7c. Comparison of almost-complete 16S rDNA sequences demonstrated that the strains were phylogenetically closely related to each other (99·5 % sequence identity), and related to Marinomonas species (94·4–96·4 % identity). DNA–DNA reassociation between KMM 3633T and KMM 3634 occurred at a level of 92 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, the name Marinomonas primoryensis sp. nov. is proposed for strains KMM 3633T and KMM 3634; the type strain is KMM 3633T (=JCM 11775T=NRIC 523T).
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