1887

Abstract

Two bacterial strains (KMM 3633 and KMM 3634) were isolated from marine coastal sea-ice and characterized by using phenotypic and molecular methods. The isolates were aerobic, Gram-negative, psychrophilic, halophilic and motile by means of a single polar flagellum. The DNA G+C content was 45·3–45·6 mol%. The major cellular fatty acids were C, C 9 and C 7. Comparison of almost-complete 16S rDNA sequences demonstrated that the strains were phylogenetically closely related to each other (99·5 % sequence identity), and related to species (94·4–96·4 % identity). DNA–DNA reassociation between KMM 3633 and KMM 3634 occurred at a level of 92 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, the name sp. nov. is proposed for strains KMM 3633 and KMM 3634; the type strain is KMM 3633 (=JCM 11775=NRIC 523).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02280-0
2003-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/3/ijs530829.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02280-0&mimeType=html&fmt=ahah

References

  1. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D. ( 1972; ). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429.
    [Google Scholar]
  2. Baumann, P., Gauthier, M. J. & Baumann, L. ( 1984; ). Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 343–352. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Gauthier, M. J. & Breittmayer, V. A. ( 1992; ). The genera Alteromonas and Marinomonas. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, vol. 3, pp. 3046–3070. Edited by A. Balows, H. G. Truper, M. Dworkin, W. Harder & K.-H. Schleifer. Berlin: Springer.
  5. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  6. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  7. Owen, R. J., Hill, L. R. & Lapage, S. P. ( 1969; ). Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7, 503–516.[CrossRef]
    [Google Scholar]
  8. Reichelt, J. L. & Baumann, P. ( 1973; ). Change of the name Alteromonas marinopraesens (ZoBell and Upham) Baumann et al. to Alteromonas haloplanktis (ZoBell and Upham) comb. nov. and assignment of strain ATCC 23821 (Pseudomonas enalia) and strain c-A1 of De Voe and Oginsky to this species. Int J Syst Bacteriol 23, 438–441.[CrossRef]
    [Google Scholar]
  9. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  10. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  11. Solano, F., García, E., Pérez de Egea, E. & Sanchez-Amat, A. ( 1997; ). Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl Environ Microbiol 63, 3499–3506.
    [Google Scholar]
  12. Solano, F. & Sanchez-Amat, A. ( 1999; ). Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49, 1241–1246.[CrossRef]
    [Google Scholar]
  13. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  14. Svetashev, V. I., Vysotskii, M. V., Ivanova, E. P. & Mikhailov, V. V. ( 1995; ). Cellular fatty acids of Alteromonas species. Syst Appl Microbiol 18, 37–43.[CrossRef]
    [Google Scholar]
  15. Van Landschoot, A. & De Ley, J. ( 1983; ). Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129, 3057–3074.
    [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02280-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02280-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error