1887

Abstract

A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31, was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus . Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97 % with (GenBank accession no: AM072820; 99.4 %), (AM040493; 98.2 %), (AJ781047; 97.8 %), (AB007419; 97.4 %), (EU346911; 97.1 %) and (AM072819; 97.1 %). In contrast, DNA–DNA hybridization experiments showed similarity values below 28 % for DNA samples from the most closely related type strains of , and . Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme II differentiated strain JG 31 from all type strains of the genus . The dominant fatty acids of the novel isolate were anteiso-C, anteiso-C and iso-C, while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31 was able to grow in a medium containing up to 300 mM KCrO and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain JG 31 ( = DSM 22788  = CCOS 200).

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2011-04-01
2020-09-30
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1987 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  2. Behrendt U., Ulrich A., Schumann P. 2008; Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L. Int J Syst Evol Microbiol 58:2574–2578 [CrossRef][PubMed]
    [Google Scholar]
  3. Bertani G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli . J Bacteriol 62:293–300[PubMed]
    [Google Scholar]
  4. Bruce J. 1996; Automated system rapidly identifies and characterizes microorganisms in food. Food Technol 50:77–81
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef][PubMed]
    [Google Scholar]
  6. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  8. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef][PubMed]
    [Google Scholar]
  9. Halpern M., Shakéd T., Pukall R., Schumann P. 2009; Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 59:665–670 [CrossRef][PubMed]
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  12. Lin Y. C., Uemori K., de Briel D. A., Arunpairojana V., Yokota A. 2004; Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae . Int J Syst Evol Microbiol 54:1669–1676 [CrossRef][PubMed]
    [Google Scholar]
  13. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160[PubMed]
    [Google Scholar]
  14. Martin E., Lodders N., Jäckel U., Schumann P., Kämpfer P. 2010; Leucobacter aerolatus sp. nov., from the air of a duck barn. Int J Syst Evol Microbiol 60:2838–2842 [CrossRef][PubMed]
    [Google Scholar]
  15. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty-acid and polar lipid-composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
    [Google Scholar]
  16. Morais P. V., Francisco R., Branco R., Chung A. P., da Costa M. S. 2004; Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 27:646–652 [CrossRef][PubMed]
    [Google Scholar]
  17. Morais P. V., Paulo C., Francisco R., Branco R., Paula Chung A., da Costa M. S. 2006; Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 29:414–421 [CrossRef][PubMed]
    [Google Scholar]
  18. Muir R. E., Tan M. W. 2007; Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens . Int J Syst Evol Microbiol 57:2770–2776 [CrossRef][PubMed]
    [Google Scholar]
  19. Rainey F. A., Dorsch M., Morgan H. W., Stackebrandt E. 1992; 16S rDNA analysis of Spirochaeta thermophila: its phylogenetic position and implications for the systematics of the order Spirochaetales . Syst Appl Microbiol 15:197–202 [CrossRef]
    [Google Scholar]
  20. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [CrossRef]
    [Google Scholar]
  21. Schuetz B., Schicklberger M., Kuermann J., Spormann A. M., Gescher J. 2009; Periplasmic electron transfer via the c-type cytochromes MtrA and FccA of Shewanella oneidensis MR-1. Appl Environ Microbiol 75:7789–7796 [CrossRef][PubMed]
    [Google Scholar]
  22. Somvanshi V. S., Lang E., Schumann P., Pukall R., Kroppenstedt R. M., Ganguly S., Stackebrandt E. 2007; Leucobacter iarius sp. nov., in the family Microbacteriaceae . Int J Syst Evol Microbiol 57:682–686 [CrossRef][PubMed]
    [Google Scholar]
  23. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  24. Takeuchi M., Weiss N., Schumann P., Yokota A. 1996; Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:967–971 [CrossRef][PubMed]
    [Google Scholar]
  25. Tóth E. M., Schumann P., Borsodi A. K., Kéki Z., Kovács A. L., Márialigeti K. 2008; Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). Int J Syst Evol Microbiol 58:976–981 [CrossRef][PubMed]
    [Google Scholar]
  26. Wayne L. G. 1988; International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Zentralbl Bakteriol Mikrobiol Hyg [A] 268:433–434[PubMed]
    [Google Scholar]
  27. Yun J. H., Roh S. W., Kim M. S., Jung M. J., Park E. J., Shin K. S., Nam Y. D., Bae J. W. 2011; Leucobacter salsicius sp. nov. from a salt-fermented food. Int J Syst Evol Microbiol 61:502–506 [CrossRef][PubMed]
    [Google Scholar]
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