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Abstract

A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31, was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus . Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97 % with (GenBank accession no: AM072820; 99.4 %), (AM040493; 98.2 %), (AJ781047; 97.8 %), (AB007419; 97.4 %), (EU346911; 97.1 %) and (AM072819; 97.1 %). In contrast, DNA–DNA hybridization experiments showed similarity values below 28 % for DNA samples from the most closely related type strains of , and . Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme II differentiated strain JG 31 from all type strains of the genus . The dominant fatty acids of the novel isolate were anteiso-C, anteiso-C and iso-C, while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31 was able to grow in a medium containing up to 300 mM KCrO and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain JG 31 ( = DSM 22788  = CCOS 200).

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2011-04-01
2019-10-20
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vol. , part 4, pp. 956 - 960

[ PDF] (68 KB) containing:

Dendrogram generated by the BioTyper software (version 2.0; Bruker Daltonics) showing the similarity of MALDI-TOF mass spectra of protein extracts of strain JG 31 and type strains of the genus .

Dendrogram showing the diversity of II RiboPrint patterns of strain JG 31 and type strains of the genus .

Polar lipid profile of strain JG 31 after two-dimensional TLC and detection with molybdatophosphoric acid.

Fatty acid composition analysis.



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