1887

Abstract

A Gram-negative, non-spore-forming, endophytic bacterium, strain YC6886, was isolated from the root of a halophyte, , which inhabits coastal areas of Namhae Island off the southern coast of Korea. Cells were non-motile, obligately aerobic rods and formed pale-yellow colonies. The isolate grew at 4–32 °C (optimum 25–28 °C) and at pH 6.5–9.5 (optimum pH 7.5) and grew optimally with 2–3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. Strain YC6886 produced yellow carotenoid pigments. Strain YC6886 exhibited the highest 16S rRNA gene sequence similarity with MN1-1037 (97.4 %). Sequence similarities between strain YC6886 and other members of the genus were 93.9–94.7 %. DNA–DNA relatedness between strain YC6886 and KCTC 22202 and KCTC 22201 was 27 and 15 %, respectively. The major fatty acids were iso-C, C and Cω9 and minor components were C, C and anteiso-C. The major respiratory quinone was menaquinone 9 and the DNA G+C content was 58.5 mol%. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and an unknown phosphoglycolipid. On the basis of phenotypic, chemotaxonomic, DNA–DNA hybridization and phylogenetic analysis, strain YC6886 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YC6886 ( = KCTC 22447  = DSM 21608). An emended description of the genus is also presented.

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2011-08-01
2019-10-20
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Absorption spectrum of an acetone extract of sp. nov. YC6886 , showing maxima for carotenoids at 340, 450 and 490 nm.

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Polar lipid profiles of sp. nov. YC6886 (a), KCTC 22202 (b) and KCTC 22201 (c) after two-dimensional TLC and detection with phosphomolybdic acid. Ascending solvent systems: (I) chloroform/methanol/water (65:25:4, by vol.); (II) chloroform/acetic acid/methanol/water (80:15:12:4, by vol.). DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; GL, unknown glycolipid; PGL, unknown phosphoglycolipid; PL, unknown phospholipid.

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