1887

Abstract

A Gram-negative, chemoheterotrophic, yellow-pigmented, non-motile, flexirubin-negative, facultatively anaerobic bacterium, designated strain IMCC1412, was isolated from a marine polychaete inhabiting a tidal flat of the Yellow Sea, Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC1412 was most closely related to , with a sequence similarity of 96.7 %, but only distantly related to other species in the family (<92 % similarity). The G+C content of the DNA was 37.3 mol%. The strain contained MK-6 as the major respiratory quinone and phosphatidylethanolamine, unidentified aminolipids and unidentified polar lipids as the major polar lipids. On the basis of phylogenetic distinctiveness and differential phenotypic characteristics, strain IMCC1412 ( = KACC 14311 = KCTC 22801 = NBRC 106077) should be assigned to the genus as the type strain of a novel species, for which the name sp. nov. is proposed. An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022764-0
2011-04-01
2020-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/864.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022764-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y.. ( 2006;). An introduction to the family Flavobacteriaceae. . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455–480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;.
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Cho J.-C., Giovannoni S. J.. ( 2003;). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. . Int J Syst Evol Microbiol 53:, 1031–1036. [CrossRef][PubMed]
    [Google Scholar]
  4. Choo Y.-J., Lee K., Song J., Cho J.-C.. ( 2007;). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. . Int J Syst Evol Microbiol 57:, 532–537. [CrossRef][PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  6. Collins M.. ( 1985;). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 121–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sohn J. H., Lee J. H., Yi H., Chun J., Bae K. S., Ahn T. Y., Kim S. J.. ( 2004;). Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. . Int J Syst Evol Microbiol 54:, 675–680. [CrossRef][PubMed]
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  16. Swofford, D. L. (2002). paup*: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates.
  17. Tindall B. J., De Vos P., Trüper H. J.. ( 2008;). Judicial Commission of the International Committee on Systematics of Prokaryotes; XIth International (IUMS) Congress of Bacteriology and Applied Microbiology. Minutes of the meetings, 23, 24 and 27 July 2005, San Francisco, CA, USA. . Int J Syst Evol Microbiol 58:, 1737–1745. [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  19. Yang S.-J., Cho J.-C.. ( 2008;). Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. . Int J Syst Evol Microbiol 58:, 315–318. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022764-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022764-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 864 - 869

Two-dimensional TLC of total polar lipids of strains IMCC1412 (a) and KCTC 8814P (b). PE, phosphatidylethanolamine; APL, unknown aminophospholipid; AL1–AL3, unknown aminolipids; L1–L3, unknown lipids.



IMAGE

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error