1887

Abstract

Two novel Gram-reaction-positive, rod-shaped actinobacterial strains, designated RP-B26 and RP-B30, were isolated from rhizosphere soil of a cliff-associated plant ( Thunb.) which was collected from Mara Island, Republic of Korea. The colonies of the isolates were circular, smooth, convex and moderately yellow–light-yellow in colour. 16S rRNA gene sequence analysis revealed that the isolates belonged to the family and formed two distinct sublineages within the radiation of the genus 16S rRNA gene sequence similarity between the isolates was 98.2 %. The closest phylogenetic neighbours of strain RP-B26 were DCY24 and A2-4 with 97.4 and 97.0 % 16S rRNA gene sequence similarity, respectively, whereas 16S rRNA gene sequence similarities between strain RP-B30 and DCY24 and A2-4 were 96.5 and 96.0 %, respectively. Both of the isolates contained -diaminopimelic acid as the diagnostic diamino acid in the cell walls. The predominant menaquinone was MK-8(H). The polar lipids were phosphatidylinositol and phosphatidylglycerol. The fatty acid profiles of the isolates were characterized by the presence of saturated, unsaturated, 10-methyl and hydroxyl fatty acids, with small amounts of branched fatty acids. The DNA G+C contents of strains RP-B26 and RP-B30 were 73.0 and 71.7 mol%, respectively. Levels of DNA–DNA relatedness between the isolates were 44.9±1.5 % (thermal renaturation method) and 43.2 % (photobiotin-labelled method); the isolates showed low DNA–DNA relatedness values (<11 %) to the most closely related strain, KCTC 19265. On the basis of the phenotypic, genotypic and DNA–DNA hybridization data presented here, the isolates are considered to represent two novel species of the genus , for which the names sp. nov. (type strain RP-B26  = KCTC 19368  = DSM 19768) and sp. nov. (type strain RP-B30  = KCTC 19384  = DSM 19769) are proposed.

Funding
This study was supported by the:
  • , 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology, Republic of Korea
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2011-08-01
2020-11-30
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