1887

Abstract

Seven isolates of an unidentified Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from domestic and wild pigs were characterized by phenotypic and molecular-genetic methods. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus , although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing showed that the unknown bacterium was phylogenetically closely related to, but distinct from, (97.5 % 16S rRNA gene sequence similarity to the type strain). and sequence analysis showed minimum interspecies divergence from phylogenetically close 16S rRNA gene sequence-based relatives of 13.8 and 18.6 %, respectively. DNA–DNA hybridization of a strain of the unidentified organism demonstrated 8–18 % reassociation with NCTC 10234. The novel bacterium could be distinguished from and other species using biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from domestic and wild animals be assigned to a novel species of the genus , sp. nov. The type strain is 682-03 ( = CCUG 58479  = CECT 7593).

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2011-07-01
2019-10-19
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Supplements

vol. , part 7, pp. 1585 - 1589

Neighbour-joining phylogenetic trees inferred from (a) and (b) gene sequences showing the position of strain 682-03 ( sp. nov.) and its closest phylogenetic neighbours.

Characteristics that differentiate sp. nov. from the main streptococcal 'species groups'.

[PDF file of Supplementary Figure and Table](215 KB)



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