1887

Abstract

Seven isolates of an unidentified Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from domestic and wild pigs were characterized by phenotypic and molecular-genetic methods. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus , although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing showed that the unknown bacterium was phylogenetically closely related to, but distinct from, (97.5 % 16S rRNA gene sequence similarity to the type strain). and sequence analysis showed minimum interspecies divergence from phylogenetically close 16S rRNA gene sequence-based relatives of 13.8 and 18.6 %, respectively. DNA–DNA hybridization of a strain of the unidentified organism demonstrated 8–18 % reassociation with NCTC 10234. The novel bacterium could be distinguished from and other species using biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from domestic and wild animals be assigned to a novel species of the genus , sp. nov. The type strain is 682-03 ( = CCUG 58479  = CECT 7593).

Funding
This study was supported by the:
  • Spanish Ministry of Education and Science (MEC) (Award AGL2009-14303-C02-01 and FAU 2006-00011-CO2-02)
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2011-07-01
2024-04-18
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References

  1. Baele M., Devriese L. A., Vancanneyt M., Vaneechoutte M., Snauwaert C., Swings J., Haesebrouck F. 2003; Emended description of Streptococcus ferus isolated from pigs and rats. Int J Syst Evol Microbiol 53:143–146 [View Article][PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  3. Chanter N. 1997; Streptococci and enterococci as animal pathogens. Soc Appl Bacteriol Symp Ser 26:Suppl.100S–109S[PubMed] [CrossRef]
    [Google Scholar]
  4. Collins M. D., Hutson R. A., Hoyles L., Falsen E., Nikolaitchouk N., Foster G. 2001; Streptococcus ovis sp. nov., isolated from sheep. Int J Syst Evol Microbiol 51:1147–1150[PubMed] [CrossRef]
    [Google Scholar]
  5. Collins M. D., Hutson R. A., Falsen E., Inganäs E., Bisgaard M. 2002; Streptococcus gallinaceus sp. nov., from chickens. Int J Syst Evol Microbiol 52:1161–1164 [View Article][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  7. Devriese L. A., Kilpper-Bälz R., Schleifer K. H. 1988; Streptococcus hyointestinalis sp. nov. from the gut of swine. Int J Syst Bacteriol 38:440–441 [View Article]
    [Google Scholar]
  8. Devriese L. A., Pot B., Vandamme P., Kersters K., Collins M. D., Alvarez N., Haesebrouck F., Hommez J. 1997; Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov., from the genital tract of sows. Int J Syst Bacteriol 47:1073–1077 [View Article][PubMed]
    [Google Scholar]
  9. Drancourt M., Roux V., Fournier P. E., Raoult D. 2004; rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella . J Clin Microbiol 42:497–504 [View Article][PubMed]
    [Google Scholar]
  10. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [CrossRef]
    [Google Scholar]
  11. Facklam R. 2002; What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15:613–630 [View Article][PubMed]
    [Google Scholar]
  12. Facklam R. R., Elliott J. A. 1995; Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8:479–495[PubMed]
    [Google Scholar]
  13. Glazunova O. O., Raoult D., Roux V. 2006; Streptococcus massiliensis sp. nov., isolated from a patient blood culture. Int J Syst Evol Microbiol 56:1127–1131[PubMed] [CrossRef]
    [Google Scholar]
  14. Glazunova O. O., Raoult D., Roux V. 2009; Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus . Int J Syst Evol Microbiol 59:2317–2322 [View Article][PubMed]
    [Google Scholar]
  15. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [View Article][PubMed]
    [Google Scholar]
  16. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  17. Kilian M. 1998; Streptococcus and Lactococcus . In Topley & Wilson’s Microbiology and Microbial Infections vol. 2 pp. 633–667 Edited by Balows A., Duerden B. I. London: Arnold;
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  19. Köhler W. 2007; The present state of species within the genera Streptococcus and Enterococcus . Int J Med Microbiol 297:133–150 [View Article][PubMed]
    [Google Scholar]
  20. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  21. Lawson P. A., Foster G., Falsen E., Collins M. D. 2005a; Streptococcus marimammalium sp. nov., isolated from seals. Int J Syst Evol Microbiol 55:271–274 [View Article][PubMed]
    [Google Scholar]
  22. Lawson P. A., Foster G. A., Falsen E., Markopoulos S. J., Collins M. D. 2005b; Streptococcus castoreus sp. nov., isolated from a beaver (Castor fiber). Int J Syst Evol Microbiol 55:843–846 [View Article][PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  24. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358[PubMed]
    [Google Scholar]
  25. Pearson W. R. 1994; Using the fasta program to search protein and DNA sequence databases. Methods Mol Biol 24:307–331[PubMed]
    [Google Scholar]
  26. Poyart C., Quesne G., Coulon S., Berche P., Trieu-Cuot P. 1998; Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 36:41–47[PubMed]
    [Google Scholar]
  27. Poyart C., Quesne G., Trieu-Cuot P. 2002; Taxonomic dissection of the Streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (sodA) sequences: reclassification of ‘Streptococcus infantarius subsp. coli’ as Streptococcus lutetiensis sp. nov. and of Streptococcus bovis biotype 11.2 as Streptococcus pasteurianus sp. nov.. Int J Syst Evol Microbiol 52:1247–1255 [View Article][PubMed]
    [Google Scholar]
  28. Rasmussen, S. W. (2002). SEQtools, a program suite for sequence analysis. http://www.seqtools.dk
  29. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  30. Takada K., Hirasawa M. 2007; Streptococcus orisuis sp. nov., isolated from the pig oral cavity. Int J Syst Evol Microbiol 57:1272–1275 [View Article][PubMed]
    [Google Scholar]
  31. Takada K., Hayashi K., Sato Y., Hirasawa M. 2010; Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity. Int J Syst Evol Microbiol 60:820–823 [View Article][PubMed]
    [Google Scholar]
  32. Vela A. I., Fernández E., Lawson P. A., Latre M. V., Falsen E., Domínguez L., Collins M. D., Fernández-Garayzábal J. F. 2002; Streptococcus entericus sp. nov., isolated from cattle intestine. Int J Syst Evol Microbiol 52:665–669[PubMed]
    [Google Scholar]
  33. Vela A. I., Casamayor A., Sánchez Del Rey V., Domínguez L., Fernández-Garayzábal J. F. 2009; Streptococcus plurextorum sp. nov., isolated from pigs. Int J Syst Evol Microbiol 59:504–508 [View Article][PubMed]
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  35. Whiley R. A., Hardie J. M. 2009; Genus I. Streptococcus Rosenbach 1884, 22AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 655–711 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. New York: Springer;
    [Google Scholar]
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