RT Journal Article SR Electronic(1) A1 Rusznyák, Anna A1 Tóth, Erika M. A1 Schumann, Peter A1 Spröer, Cathrin A1 Makk, Judit A1 Szabó, Gitta A1 Vladár, Péter A1 Márialigeti, Károly A1 Borsodi, Andrea K.YR 2011 T1 Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 7 SP 1662 OP 1666 DO https://doi.org/10.1099/ijs.0.022608-0 PB Microbiology Society, SN 1466-5034, AB An alkalitolerant and moderately halophilic strain, designated KB23T, characterized by optimal growth at pH 8.0–9.0 and in the presence of 5–7 % (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23T were Gram-stain-positive, motile straight rods. Strain KB23T was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (l-Orn–d-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C15 : 0, C16 : 0 and anteiso-C15 : 1 were the major cellular fatty acids. The DNA G+C content of strain KB23T was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109T (97.35 % similarity), Cellulomonas terrae DB5T (96.81 %), Cellulomonas iranensis OT (96.75), Cellulomonas chitinilytica X.bu-bT (96.60 %), Cellulomonas persica IT (96.53 %), Cellulomonas composti TR7-06T (96.45 %), Cellulomonas biazotea DSM 20112T (96.34 %) and Cellulomonas fimi DSM 20113T (96.20 %). According to these results, together with DNA–DNA hybridization and physiological data, strain KB23T is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23T ( = DSM 22512T  = NCAIM B002303T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.022608-0