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Abstract

An alkalitolerant and moderately halophilic strain, designated KB23, characterized by optimal growth at pH 8.0–9.0 and in the presence of 5–7 % (w/v) NaCl, was isolated from a reed () periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23 were Gram-stain-positive, motile straight rods. Strain KB23 was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (-Orn–-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C, C and anteiso-C were the major cellular fatty acids. The DNA G+C content of strain KB23 was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus and that it is related most closely to DSM 20109 (97.35 % similarity), DB5 (96.81 %), O (96.75), X.bu-b (96.60 %), I (96.53 %), TR7-06 (96.45 %), DSM 20112 (96.34 %) and DSM 20113 (96.20 %). According to these results, together with DNA–DNA hybridization and physiological data, strain KB23 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KB23 ( = DSM 22512  = NCAIM B002303).

Funding
This study was supported by the:
  • Hungarian Scientific Research Fund (Award T038021)
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2011-07-01
2022-01-21
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