1887

Abstract

A Gram-stain-positive, aerobic, non-motile actinomycete strain, designated YIM 65601, was studied by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain YIM 65601 should be assigned to the genus , with highest 16S rRNA gene sequence similarities to HMC10 (98.8 %), DSM 43678 (98.7 %), . DSM 43926 (98.5 %), DSM 43767 (98.4 %), IFO 14309 (98.2 %) and GW 14-1925 (98.1 %). 16S rRNA gene sequence similarities to strains of other species were below 98.0 %. Morphological and chemotaxonomic properties of the isolate were consistent with those of members of the genus . The results of DNA–DNA hybridization and physiological and biochemical tests and fatty acid profiles allowed genotypic and phenotypic differentiation of strain YIM 65601 from closely related species. Thus, YIM 65601 represents a novel species of the genus , for which the name sp. nov. is proposed, with YIM 65601 ( = CCTCC AA 209037  = DSM 45385) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022558-0
2011-04-01
2020-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/757.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022558-0&mimeType=html&fmt=ahah

References

  1. Chiba S. , Suzuki M. , Ando K. . ( 1999; ). Taxonomic re-evaluation of ‘Nocardiopsis’ sp. K-252T ( = NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov.. Int J Syst Bacteriol 49:, 1623–1630. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470.[CrossRef]
    [Google Scholar]
  4. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fischer A. , Kroppenstedt R. M. , Stackebrandt E. . ( 1983; ). Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis . . J Gen Microbiol 129:, 3433–3446.[PubMed]
    [Google Scholar]
  9. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  10. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  11. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  12. He L. , Li W. , Huang Y. , Wang L. M. , Liu Z. H. , Lanoot B. J. , Vancanneyt M. , Swings J. . ( 2005; ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. . Int J Syst Evol Microbiol 55:, 1939–1944. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kämpfer P. , Kroppenstedt R. M. , Grün-Wollny I. . ( 2005; ). Nonomuraea kuesteri sp. nov.. Int J Syst Evol Microbiol 55:, 847–851. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kämpfer P. , Busse H. J. , Tindall B. J. , Nimtz M. , Grün-Wollny I. . ( 2010; ). Nonomuraea rosea sp. nov.. Int J Syst Evol Microbiol 60:, 1118–1124. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kelly K. L. . ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  16. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  17. Li W. J. , Xu P. , Schumann P. , Zhang Y. Q. , Pukall R. , Xu L. H. , Stackebrandt E. , Jiang C. L. . ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95.[CrossRef]
    [Google Scholar]
  20. Poscher J. , Kroppenstedt R. M. , Fischer A. , Stackebrandt E. . ( 1985; ). DNA : DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis . . Syst Appl Microbiol 6:, 264–270.[CrossRef]
    [Google Scholar]
  21. Qin S. , Li J. , Chen H. H. , Zhao G. Z. , Zhu W. Y. , Jiang C. L. , Xu L. H. , Li W. J. . ( 2009; ). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. . Appl Environ Microbiol 75:, 6176–6186. [CrossRef] [PubMed]
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  24. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  25. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  26. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  27. Stackebrandt E. , Wink J. , Steiner U. , Kroppenstedt R. M. . ( 2001; ). Nonomuraea dietzii sp. nov.. Int J Syst Evol Microbiol 51:, 1437–1441.[PubMed]
    [Google Scholar]
  28. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Microbiol 54:, 31–36.
    [Google Scholar]
  29. Tamura T. , Suzuki S. , Hatano K. . ( 2000; ). Acrocarpospora gen. nov., a new genus of the order Actinomycetales . . Int J Syst Evol Microbiol 50:, 1163–1171.[PubMed] [CrossRef]
    [Google Scholar]
  30. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  31. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  32. Waksman S. A. . ( 1961; ). The Actinomycetes, vol. 2. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  33. Zhang Z. , Wang Y. , Ruan J. . ( 1998; ). Reclassification of Thermomonospora and Microtetraspora . . Int J Syst Bacteriol 48:, 411–422.[PubMed] [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022558-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022558-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error