1887

Abstract

An aerobic, Gram-negative, non-fermentative, rod-shaped, motile, non-pigmented bacterium, KMM 1406, was isolated from a sample of Pacific deep sea water and investigated for phenotypic characteristics, chemotaxonomic features and phylogenetic relationships. The deep-sea isolate exhibited growth in 0–8 % (w/v) NaCl and at 4–37 °C, hydrolytic activity on gelatin, Tween 80 and starch and lack of -glucose utilization. The major fatty acids were C, C 9, C 8 and C 7. The DNA G+C content was 49·6 mol%. 16S rRNA gene sequence analysis revealed that strain KMM 1406 was related closely to DSM 14885 within the -, with 96·8 % sequence similarity. On the basis of phenotypic and molecular data, a novel species, sp. nov., is proposed. The type strain is KMM 1406 (=IAM 15043=JCM 12090=NRIC 0539=CCUG 46544).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02252-0
2003-11-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/6/ijs531973.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02252-0&mimeType=html&fmt=ahah

References

  1. Brettar, I., Christen, R. & Höfle, M. G. ( 2002; ). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52, 1851–1857.[CrossRef]
    [Google Scholar]
  2. Fonnesbech Vogel, B., Venkateswaran, K., Christensen, H., Falsen, E., Christiansen, G. & Gram, L. ( 2000; ). Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50, 1133–1142.[CrossRef]
    [Google Scholar]
  3. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  4. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  5. Owen, J., Hill, L. R. & Lapage, S. P. ( 1969; ). Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7, 503–516.[CrossRef]
    [Google Scholar]
  6. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  7. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  8. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  9. Svetashev, V. I., Vysotskii, M. V., Ivanova, E. P. & Mikhailov, V. V. ( 1995; ). Cellular fatty acids of Alteromonas species. Syst Appl Microbiol 18, 37–43.[CrossRef]
    [Google Scholar]
  10. Takami, H., Kobata, K., Nagahama, T., Kobayashi, H., Inoue, A. & Horikoshi, K. ( 1999; ). Biodiversity in deep-sea sites located near the south part of Japan. Extremophiles 3, 97–102.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02252-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02252-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error