1887

Abstract

A Gram-positive bacterium, strain M-6-3, was isolated from salt-fermented seafood in Korea. The organism grew in 0–10 % (w/v) NaCl and at 25–37 °C, with optimal growth occurring in 5 % NaCl and at 28–30 °C. The peptidoglycan type was variation A4γ with -diaminopimelic acid as the diagnostic cell-wall diamino acid. The polar lipid profile of strain M-6-3 consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. Strain M-6-3 contained MK-7 as the major component of the quinone system and anteiso-C (62.1 %) as the predominant fatty acid. Based on 16S rRNA gene sequence similarity studies, strain M-6-3 was most closely related to LMG 19848 (98.5 %). The G+C content of the genomic DNA was 71.5 mol% and the mean DNA–DNA hybridization value with reference strains was 14.32±2.0 %. Based on phenotypic, genotypic and phylogenetic analyses, it is proposed that strain M-6-3 represents a novel species for which the name sp. nov. is proposed; the type strain is M-6-3 ( = KACC 14221  = JCM 16464).

Funding
This study was supported by the:
  • , TDPAF (Technology Development Program for Agriculture 160 and Forestry)
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2011-05-01
2021-01-22
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References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. 2005; Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. Appl Environ Microbiol 71:8825–8835 [CrossRef][PubMed]
    [Google Scholar]
  2. Baker G. C., Smith J. J., Cowan D. A. 2003; Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55:541–555 [CrossRef][PubMed]
    [Google Scholar]
  3. Buczolits S., Schumann P., Weidler G., Radax C., Busse H.-J. 2003; Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int J Syst Evol Microbiol 53:1955–1960 [CrossRef][PubMed]
    [Google Scholar]
  4. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. 2008; Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 75:523–530 [CrossRef][PubMed]
    [Google Scholar]
  5. Chou J. H., Lin K. Y., Lin M. C., Sheu S. Y., Wei Y. H., Arun A. B., Young C. C., Chen W. M. 2007; Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. Int J Syst Evol Microbiol 57:2674–2679 [CrossRef][PubMed]
    [Google Scholar]
  6. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  7. Collins M. D., Brown J., Jones D. 1988; Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int J Syst Bacteriol 38:45–48 [CrossRef]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [CrossRef][PubMed]
    [Google Scholar]
  9. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol 47:788–794 [CrossRef][PubMed]
    [Google Scholar]
  10. Gvozdyak O. R., Nogina T. M., Schumann P. 1992; Taxonomic study of the genus Brachybacterium: Brachybacterium nesterenkovii sp. nov.. Int J Syst Bacteriol 42:74–78 [CrossRef][PubMed]
    [Google Scholar]
  11. Heyrman J., Balcaen A., De Vos P., Schumann P., Swings J. 2002; Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Evol Microbiol 52:1641–1646 [CrossRef][PubMed]
    [Google Scholar]
  12. Holding A. J., Collee J. G. 1971; Routine biochemical tests. Methods Microbiol 6A:1–32 [CrossRef]
    [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  14. Loy A., Schulz C., Lücker S., Schöpfer-Wendels A., Stoecker K., Baranyi C., Lehner A., Wagner M. 2005; 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales”. Appl Environ Microbiol 71:1373–1386 [CrossRef][PubMed]
    [Google Scholar]
  15. MIDI 1999 Sherlock Microbial Identification System Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  18. Schubert K., Ludwig W., Springer N., Kroppenstedt R. M., Accolas J. P., Fiedler F. 1996; Two coryneform bacteria isolated from the surface of French Gruyère and Beaufort cheeses are new species of the genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov.. Int J Syst Bacteriol 46:81–87 [CrossRef][PubMed]
    [Google Scholar]
  19. Takeuchi M., Fang C.-X., Yokota A. 1995; Taxonomic study of the genus Brachybacterium: proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov.. Int J Syst Bacteriol 45:160–168 [CrossRef]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  23. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. 2000; Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303[PubMed] [CrossRef]
    [Google Scholar]
  24. Zhang G., Zeng G., Cai X., Deng S., Luo H., Sun G. 2007; Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China. Int J Syst Evol Microbiol 57:2519–2524 [CrossRef][PubMed]
    [Google Scholar]
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