1887

Abstract

A Gram-positive bacterium, strain M-6-3, was isolated from salt-fermented seafood in Korea. The organism grew in 0–10 % (w/v) NaCl and at 25–37 °C, with optimal growth occurring in 5 % NaCl and at 28–30 °C. The peptidoglycan type was variation A4γ with -diaminopimelic acid as the diagnostic cell-wall diamino acid. The polar lipid profile of strain M-6-3 consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. Strain M-6-3 contained MK-7 as the major component of the quinone system and anteiso-C (62.1 %) as the predominant fatty acid. Based on 16S rRNA gene sequence similarity studies, strain M-6-3 was most closely related to LMG 19848 (98.5 %). The G+C content of the genomic DNA was 71.5 mol% and the mean DNA–DNA hybridization value with reference strains was 14.32±2.0 %. Based on phenotypic, genotypic and phylogenetic analyses, it is proposed that strain M-6-3 represents a novel species for which the name sp. nov. is proposed; the type strain is M-6-3 ( = KACC 14221  = JCM 16464).

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2011-05-01
2019-12-07
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References

  1. Bae J. W. , Rhee S. K. , Park J. R. , Chung W. H. , Nam Y. D. , Lee I. , Kim H. , Park Y. H. . ( 2005; ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71:, 8825–8835. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baker G. C. , Smith J. J. , Cowan D. A. . ( 2003; ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55:, 541–555. [CrossRef] [PubMed]
    [Google Scholar]
  3. Buczolits S. , Schumann P. , Weidler G. , Radax C. , Busse H.-J. . ( 2003; ). Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. . Int J Syst Evol Microbiol 53:, 1955–1960. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chang H. W. , Nam Y. D. , Jung M. Y. , Kim K. H. , Roh S. W. , Kim M. S. , Jeon C. O. , Yoon J. H. , Bae J. W. . ( 2008; ). Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75:, 523–530. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chou J. H. , Lin K. Y. , Lin M. C. , Sheu S. Y. , Wei Y. H. , Arun A. B. , Young C. C. , Chen W. M. . ( 2007; ). Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. . Int J Syst Evol Microbiol 57:, 2674–2679. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  7. Collins M. D. , Brown J. , Jones D. . ( 1988; ). Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. . Int J Syst Bacteriol 38:, 45–48.[CrossRef]
    [Google Scholar]
  8. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef] [PubMed]
    [Google Scholar]
  9. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997; ). Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. . Int J Syst Bacteriol 47:, 788–794. [CrossRef] [PubMed]
    [Google Scholar]
  10. Gvozdyak O. R. , Nogina T. M. , Schumann P. . ( 1992; ). Taxonomic study of the genus Brachybacterium: Brachybacterium nesterenkovii sp. nov.. Int J Syst Bacteriol 42:, 74–78. [CrossRef] [PubMed]
    [Google Scholar]
  11. Heyrman J. , Balcaen A. , De Vos P. , Schumann P. , Swings J. . ( 2002; ). Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). . Int J Syst Evol Microbiol 52:, 1641–1646. [CrossRef] [PubMed]
    [Google Scholar]
  12. Holding A. J. , Collee J. G. . ( 1971; ). Routine biochemical tests. . Methods Microbiol 6A:, 1–32. [CrossRef]
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Loy A. , Schulz C. , Lücker S. , Schöpfer-Wendels A. , Stoecker K. , Baranyi C. , Lehner A. , Wagner M. . ( 2005; ). 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales”. . Appl Environ Microbiol 71:, 1373–1386. [CrossRef] [PubMed]
    [Google Scholar]
  15. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  16. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  18. Schubert K. , Ludwig W. , Springer N. , Kroppenstedt R. M. , Accolas J. P. , Fiedler F. . ( 1996; ). Two coryneform bacteria isolated from the surface of French Gruyère and Beaufort cheeses are new species of the genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov.. Int J Syst Bacteriol 46:, 81–87. [CrossRef] [PubMed]
    [Google Scholar]
  19. Takeuchi M. , Fang C.-X. , Yokota A. . ( 1995; ). Taxonomic study of the genus Brachybacterium: proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov.. Int J Syst Bacteriol 45:, 160–168. [CrossRef]
    [Google Scholar]
  20. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  21. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tindall B. J. . ( 1990; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  23. Xin H. , Itoh T. , Zhou P. , Suzuki K. , Kamekura M. , Nakase T. . ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. . Int J Syst Evol Microbiol 50:, 1297–1303.[PubMed] [CrossRef]
    [Google Scholar]
  24. Zhang G. , Zeng G. , Cai X. , Deng S. , Luo H. , Sun G. . ( 2007; ). Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China. . Int J Syst Evol Microbiol 57:, 2519–2524. [CrossRef] [PubMed]
    [Google Scholar]
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vol. , part 5, pp. 1118 - 1122

Two-dimensional thin-layer chromatograms of phospholipids (a) and glycolipids (b) from strain M-6-3 . Solvent 1 (chloroform/methanol/water, 65 : 25 : 4, by vol.) was used in the first dimension and solvent 2 (chloroform/methanol/acetate/water, 80 : 12 : 15 : 4, by vol.) was used in the second dimension. Phospholipids were revealed by spraying the chromatograms with Zinzadze reagent and glycolipids were revealed by spraying the chromatograms with α-naphthol-sulphuric acid. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PL, unidentified phospholipid; GL, unknown glycolipid.

DNA–DNA hybridization results for strain M-6-3 with other members of the genus

Strains: 1, sp. nov. M-6-3 ; 2, DSM 10240 ; 3, DSM 15460 ; 4, DSM 9573 ; 5, LMG 20345 ; 6, LMG 20336 ; 7, JB ; 8, LMG 19861 ; 9, DSM 4810 ; 10, CNRZ 925 ; 11, phenol-A .



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