sp. nov., isolated from mangrove soil Free

Abstract

A novel actinomycete, designated strain 06182M-1, was isolated from a mangrove soil sample collected from Chiayi County in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.8 % to the type strains of recognized species of the genus . Chemotaxonomic data also supported the placement of strain 06182M-1 within the genus . However, the low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus , in combination with differential phenotypic data, demonstrate that strain 06182M-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 06182M-1 ( = BCRC 16888  = KCTC 19740).

Funding
This study was supported by the:
  • Ministry of Economic Affairs, ROC (Award 95-EC-17-A-17-R7-0525)
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2011-07-01
2024-03-29
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References

  1. Bakalidou A., Kämpfer P., Berchtold M., Kuhnigk T., Wenzel M., König H. 2002; Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis . Int J Syst Evol Microbiol 52:1185–1192 [View Article][PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Felsenstein J. 1993; phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington; Seattle, USA:
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  7. Groth I., Schumann P., Schütze B., Gonzalez J. M., Laiz L., Saiz-Jimenez C., Stackebrandt E. 2005; Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719 [View Article][PubMed]
    [Google Scholar]
  8. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
    [Google Scholar]
  9. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
    [Google Scholar]
  10. Itoh T., Kudo T., Parenti F., Seino A. 1989; Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [View Article]
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  13. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204[PubMed] [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  15. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  18. Stackebrandt E., Schumann P., Cui X.-L. 2004; Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:685–688 [View Article][PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  23. Wu Y., Li W.-J., Tian W., Zhang L.-P., Xu L., Shen Q.-R., Shen B. 2010; Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium. Int J Syst Evol Microbiol 60:904–908 [View Article][PubMed]
    [Google Scholar]
  24. Zhang Y. Q., Schumann P., Li W. J., Chen G. Z., Tian X. P., Stackebrandt E., Xu L. H., Jiang C. L. 2005; Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870 [View Article][PubMed]
    [Google Scholar]
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