1887

Abstract

is a mutans-like streptococcus originally isolated from wild rats fed with sugar cane. Taxonomically, this species has not been studied extensively. Ten Gram-positive coccal strains, isolated from the tonsils and nasal conchae of piglets, were identified as by 16S rDNA sequencing, tRNA-intergenic spacer length polymorphism analysis (tDNA-PCR), whole-cell protein profiling using SDS-PAGE, G+C content determination and extensive biochemical testing. In all these tests, the type strain of (LMG 16520), from a rat, was included. The results of the tests are described and an emended species description is presented.

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2003-01-01
2024-03-28
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References

  1. Baele M., Baele P., Vaneechoutte M., Storms V., Butaye P., Devriese L. A., Verschraegen G., Gillis M., Haesebrouck F. 2000; Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38:4201–4207
    [Google Scholar]
  2. Baele M., Chiers K., Devriese L. A., Smith H. E., Wisselink H. J., Vaneechoutte M., Haesebrouck F. 2001a; The Gram-positive tonsillar and nasal flora of piglets before and after weaning. J Appl Microbiol 91:997–1003 [CrossRef]
    [Google Scholar]
  3. Baele M., Storms V., Haesebrouck F., Devriese L. A., Gillis M., Verschraegen G., De Baere T., Vaneechoutte M. 2001b; Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species. J Clin Microbiol 39:1436–1442 [CrossRef]
    [Google Scholar]
  4. Beighton D., Hayday H., Russell R. R. B., Whiley R. A. 1984; Streptococcus macacae sp. nov. from dental plaque of monkeys ( Macaca fascicularis . Int J Syst Bacteriol 34:332–335 [CrossRef]
    [Google Scholar]
  5. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol 41:487–494 [CrossRef]
    [Google Scholar]
  6. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  7. Coykendall A. L. 1977; Proposal to elevate the subspecies of Streptococcus mutans to species status, based on their molecular composition. Int J Syst Bacteriol 27:26–30 [CrossRef]
    [Google Scholar]
  8. Coykendall A. L. 1983; Streptococcus sobrinus nom. rev. and Streptococcus ferus nom. rev.: habitat of these and other mutans streptococci. Int J Syst Bacteriol 33:883–885 [CrossRef]
    [Google Scholar]
  9. De Gheldre Y., Vandamme P., Goossens H., Struelens M. J. 1999; Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism. Int J Syst Bacteriol 49:1591–1598 [CrossRef]
    [Google Scholar]
  10. Freedman M. L., Coykendall A. L., O'Neill E. M. 1982; Physiology of ‘mutans-like’ Streptococcus ferus from wild rats. Infect Immun 35:476–482
    [Google Scholar]
  11. Hardie J. M., Whiley R. A. 1995; The genus Streptococcus . In The Genera of the Lactic Acid Bacteria pp 55–124Edited by Wood B. J. B., Holzapfel W. H. London: Blackie Academic and Professional;
    [Google Scholar]
  12. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  13. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp 493–521Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  14. Vancanneyt M., Snauwaert C., Cleenwerck I.8 other authors 2001; Enterococcus villorum sp. nov., an enteroadherent bacterium associated with diarrhoea in piglets. Int J Syst Evol Microbiol 51:393–400
    [Google Scholar]
  15. Whiley R. A., Beighton D. 1998; Current classification of the oral streptococci. Oral Microbiol Immunol 13:195–216 [CrossRef]
    [Google Scholar]
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