1887

Abstract

Two soil isolates, strains 80-60 and 3.24, were shown to have chemical and morphological properties consistent with their classification in the genus . The almost complete 16S rDNA sequences generated for the two organisms were aligned with available sequences of representatives of the genus and related taxa. It was apparent from the resultant phylogenetic trees that each of the strains formed a distinct phyletic line within the evolutionary radiation occupied by the genus . The two organisms could also be distinguished from one another and from representatives of all the validly described species of using a set of phenotypic properties. It is proposed that strains 3.24 (=AS 4.1522 =IFO 16341) and 80-60 (=AS 4.1202 =IFO 14668 =JCM 16161) be classified in the genus as sp. nov. and sp. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02243-0
2003-01-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/1/ijs530137.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02243-0&mimeType=html&fmt=ahah

References

  1. Athalye, M., Goodfellow, M., Lacey, J. & White, R. P. ( 1985; ). Numerical classification of Actinomadura and Nocardiopsis. Int J Syst Bacteriol 35, 86–98.[CrossRef]
    [Google Scholar]
  2. Couch, J. N. ( 1963; ). Some new genera and species of Actinoplanaceae. J Elisha Mitchell Sci Soc 79, 53–70.
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 143–153.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogenetic inference package), version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, WA, USA.
  5. Fischer, A., Kroppenstedt, R. M. & Stackebrandt, E. ( 1983; ). Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis. J Gen Microbiol 129, 3433–3446.
    [Google Scholar]
  6. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  7. Goodfellow, M., Stanton, L. J., Simpson, K. E. & Minnikin, D. E. ( 1990; ). Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136, 19–36.[CrossRef]
    [Google Scholar]
  8. Henssen, A. ( 1957; ). Beiträge zür Morphologie and Systematik der thermophilen Actinomyceten. Arch Mikrobiol 26, 373–414.[CrossRef]
    [Google Scholar]
  9. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. & Williams, S. T. (editors) ( 1994; ). Bergey's Manual of Determinative Bacteriology, 9th edn. Baltimore: Williams & Wilkins.
  10. Iinuma, S., Yokota, A., Hasegawa, T. & Kanamaru, T. ( 1994; ). Actinocorallia gen. nov., a new genus of the order Actinomycetales. Int J Syst Bacteriol 44, 230–234.[CrossRef]
    [Google Scholar]
  11. Itoh, T., Kudo, T. & Seino, A. ( 1987; ). Chemotaxonomic studies on new genera of actinomycetes proposed in Chinese papers. Actinomycetologica 1, 43–59.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Kawato, M. & Shinobu, R. ( 1959; ). Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Univ Lib Arts Educ 8, 114.
    [Google Scholar]
  14. Kim, D., Chun, J., Sahin, N., Hah, Y. C. & Goodfellow, M. ( 1996; ). Analysis of thermophilic clades within the genus Streptomyces by 16S ribosomal DNA sequence comparisons. Int J Syst Bacteriol 46, 581–587.[CrossRef]
    [Google Scholar]
  15. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M. ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef]
    [Google Scholar]
  16. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  17. Kroppenstedt, R. M., Stackebrandt, E. & Goodfellow, M. ( 1990; ). Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora. Syst Appl Microbiol 13, 148–160.[CrossRef]
    [Google Scholar]
  18. Lechevalier, H. A. & Lechevalier, M. P. ( 1968; ). A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, pp. 393–405. Edited by H. Prauser. Jena: Gustav Fischer.
  19. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  20. Lipski, A. & Altendorf, K. ( 1995; ). Actinomadura nitritigenes sp. nov., isolated from experimental biofilters. Int J Syst Bacteriol 45, 717–723.[CrossRef]
    [Google Scholar]
  21. Lu, Z., Liu, Z., Wang, L., Zhang, Y., Qi, W. & Goodfellow, M. ( 2001; ). Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325.
    [Google Scholar]
  22. Meyer, J. ( 1976; ). Nocardiopsis, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 487–493.[CrossRef]
    [Google Scholar]
  23. Meyer, J. ( 1979; ). New species of the genus Actinomadura. Zentbl Allg Mikrobiol 19, 37–44.[CrossRef]
    [Google Scholar]
  24. Miyadoh, S. & Miyara, T. ( 2001; ). Family Thermomonosporaceae. In Identification Manual of Actinomycetes, pp. 281–291. Edited by the Society for Actinomycetes, Japan. Tokyo: Business Center for Academic Societies.
  25. Mordarska, H., Mordarski, M. & Goodfellow, M. ( 1972; ). Chemotaxonomic characters and classification of some nocardioform bacteria. J Gen Microbiol 71, 77–86.[CrossRef]
    [Google Scholar]
  26. Ochi, K., Miyadoh, S. & Tamura, T. ( 1991; ). Polyacrylamide gel electrophoresis analysis of ribosomal protein AT-L30 as a novel approach to actinomycete taxonomy: application to the genera Actinomadura and Microtetraspora. Int J Syst Bacteriol 41, 234–239.[CrossRef]
    [Google Scholar]
  27. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  28. Poscher, J., Kroppenstedt, R. M., Fischer, A. & Stackebrandt, E. ( 1985; ). DNA : DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis. Syst Appl Microbiol 6, 264–270.[CrossRef]
    [Google Scholar]
  29. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  30. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  31. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  32. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  33. Trujillo, M. E. & Goodfellow, M. ( 1997; ). Polyphasic taxonomic study of clinically significant actinomadurae including the description of Actinomadura latina sp. nov. Zentbl Bakteriol 285, 212–233.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  35. Zhang, G., Xing, G. & Yan, X. ( 1984; ). Studies of classification of Streptomycetaceae. III. A new genus – Streptomycoides in the Streptomycetaceae. Acta Microbiol Sin 24, 189–194.
    [Google Scholar]
  36. Zhang, Z., Wang, Y. & Ruan, J. ( 1998; ). Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol 48, 411–422.[CrossRef]
    [Google Scholar]
  37. Zhang, Z., Kudo, T., Nakajima, Y. & Wang, Y. ( 2001; ). Clarification of the relationship between the members of the family Thermomonosporaceae on the basis of 16S rDNA, 16S–23S rRNA internal transcribed spacer and 23S rDNA sequences and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 373–383.
    [Google Scholar]
  38. Zhou, Z.-H., Liu, Z.-H., Qian, Y.-D., Kim, S. B. & Goodfellow, M. ( 1998; ). Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02243-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02243-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error