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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 2

sp. nov., isolated from Marine Sediment Free

Abstract

A Gram-negative, neutrophilic and rod-shaped bacterium, strain ZH17, was isolated from a marine sediment of the East China Sea and subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0–7.5 % (w/v) NaCl and at pH 6.5–9.0; optimum growth was observed with 0.5–3.0 % (w/v) NaCl and at pH 7.5. Chemotaxonomic analysis showed ubiquinone-10 as predominant respiratory quinone and C 7, 11-methyl C 7, C, C 3-OH and C 2-OH as major fatty acids. The genomic DNA G+C content was 63.5 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus . Strain ZH17 exhibited the closest phylogenetic affinity to the type strain of , with 97.2 % sequence similarity, and less than 97 % sequence similarity with respect to other described species of the genus . The DNA–DNA reassociation value between strain ZH17 and DSM 15171 was 50.7 %. On the basis of phenotypic and genotypic data, strain ZH17 represents a novel species of the genus , for which the name sp. nov. (type strain ZH17 =CGMCC 1.9108 =JCM 16262) is proposed.

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2011-02-01
2024-04-19
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References

  1. Arahal D. R., Macián M. C., Garay E., Pujalte M. J. 2005; Thalassobius mediterraneus gen. nov., sp. nov. and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55:2371–2376 [CrossRef]
     [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
     [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
     [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
     [Google Scholar]
  6. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A. other authors 2003 Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments Int J Syst Evol Microbiol 53:1261–1269 [CrossRef]
     [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
     [Google Scholar]
  8. Kates M. 1986; In Techniques of Lipidology. , 2nd rev. edn. pp. 106–107, 187–188 and 251–254 Amsterdam: Elsevier;
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  11. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
     [Google Scholar]
  12. Lai Q., Yuan J., Li F., Zheng T., Shao Z. 2010; Ruegeria pelagia is a later synonym of Ruegeria mobilis . Int J Syst Evol Microbiol 60:1918–1920 [CrossRef]
     [Google Scholar]
  13. Lee K., Choo Y.-J., Giovannoni S. J., Cho J.-C. 2007; Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean. Int J Syst Evol Microbiol 57:1815–1818 [CrossRef]
     [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
     [Google Scholar]
  15. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. 2006; Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al . 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995)Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter , Ruegeria and Leisingera . Int J Syst Evol Microbiol 56:1293–1304 [CrossRef]
     [Google Scholar]
  16. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
     [Google Scholar]
  17. Muramatsu Y., Uchino Y., Kasai H., Suzuki K., Nakagawa Y. 2007; Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57:1304–1309 [CrossRef]
     [Google Scholar]
  18. Petursdottir S. K., Kristjansson J. K. 1997; Silicibacter lacuscaerulensis gen. nov., sp. nov. a mesophilic moderately halophilic bacterium characteristic of the Blue Lagoon geothermal lake in Iceland. Extremophiles 1:94–99 [CrossRef]
     [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  22. Uchino Y., Hirata A., Yokota A., Sugiyama J. 1998; Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen.nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov.,Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44201–210 [CrossRef]
     [Google Scholar]
  23. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Faimali M., Vandamme P. 2008; Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 58:2726–2733 [CrossRef]
     [Google Scholar]
  24. Vaskovsky V. E., Kostetsky E. Y. 1968; Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 9: 396
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  26. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. 2007 Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57, 1619–1624. [CrossRef]
  27. Yi H., Lim Y. W., Chun J. 2007; Taxonomic evaluation of the genera Ruegeria and Silicibacter : a proposal to transfer the genus Silicibacter Petursdottir and Kristjansson 1999 to the genus Ruegeria Uchino et al. 1999. Int J Syst Evol Microbiol 57:815–819 [CrossRef]
     [Google Scholar]
  28. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
     [Google Scholar]
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Ruegeria marina sp. nov., isolated from Marine Sediment
Int J Syst Evol Microbiol 61, 347 (2011); https://doi.org/10.1099/ijs.0.022400-0
/content/journal/ijsem/10.1099/ijs.0.022400-0
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