sp. nov., isolated from a freshwater pond, and emended description of the genus Free

Abstract

A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2D, isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus (, ). 16S rRNA gene sequence similarities between the isolate and MWH-C5, representing the type species of the genus, and the type strains of and were 98.2, 96.5 and 97.0 %, respectively. DNA–DNA reassociation analyses with DNA of the type strains of all three previously described species revealed similarity values in the range 26.2–44.6 %. The predominant fatty acids of the isolate were C 7/6, C, C and C 3-OH, the major quinone was ubiquinone Q-8 and the DNA G+C content was 55.8 mol%. The isolate could be discriminated from the type strains of the three species by several phenotypic traits including differences in the utilization of several carbon sources. Based on the phylogeny of the isolate and its differences from the three most closely related species, the isolate represents a novel species for which the name sp. nov. is proposed. The type strain is MWH-BRAZ-DAM2D (=DSM 21646=CCUG 56719).

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2010-12-01
2024-03-19
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