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Abstract

A taxonomic study was carried out on a novel bacterial strain, designated W11-5, which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase- and catalase-positive. Growth was observed in 0.5–12 % (w/v) NaCl and at 10–42 °C. On the basis of 16S rRNA gene sequence analysis, strain W11-5 was shown to belong to the genus with a close relation to B-5 (93.9 % 16S rRNA sequence similarity), MACL04 (93.1 %), A-11-3 (93.1 %), SK2 (93.0 %), ISO4 (93.0 %) and T9 (92.9 %). Similarities between the gene sequences of W11-5 and other species of the genus were between 76.8 and 80.8 %. The principal fatty acids were C 3-OH (8.0 %), C (29.1 %) and Cω7 (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gene sequence analyses, strain W11-5 ( = MCCC 1A00474  = CCTCC AB 208236  = LMG 25514) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2011-06-01
2019-10-22
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References

  1. Ausubel F. M. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Bruns A. , Berthe-Corti L. . ( 1999; ). Fundibacter jadensis gen. nov., sp. nov., a new slightly halophilic bacterium, isolated from intertidal sediment. . Int J Syst Bacteriol 49:, 441–448. [CrossRef].[PubMed]
    [Google Scholar]
  3. Dong X.-Z. , Cai M.-Y. . (2001). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation)..
  4. Fernández-Martínez J. , Pujalte M. J. , García-Martínez J. , Mata M. , Garay E. , Rodríguez-Valeral F. . ( 2003; ). Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax . . Int J Syst Evol Microbiol 53:, 331–338. [CrossRef].[PubMed]
    [Google Scholar]
  5. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef]
    [Google Scholar]
  6. Lai Q. , Yuan J. , Wang B. , Sun F. , Qiao N. , Zheng T. , Shao Z. . ( 2009; ). Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. . Int J Syst Evol Microbiol 59:, 1579–1582. [CrossRef].[PubMed]
    [Google Scholar]
  7. Liu C. , Shao Z. . ( 2005; a). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55:, 1181–1186. [CrossRef].[PubMed]
    [Google Scholar]
  8. Liu C. , Shao Z. . ( 2005; b). Isolation and characterization of oil-degrading marine micro-organisms. . Acta Oceanol Sin 27:, 114–120.
    [Google Scholar]
  9. Mesbah M. , Whitman W. B. . ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef].[PubMed]
    [Google Scholar]
  10. Rivas R. , García-Fraile P. , Peix A. , Mateos P. F. , Martínez-Molina E. , Velázquez E. . ( 2007; ). Alcanivorax balearicus sp. nov., isolated from Lake Martel. . Int J Syst Evol Microbiol 57:, 1331–1335. [CrossRef].[PubMed]
    [Google Scholar]
  11. Rzhetsky A. , Nei M. . ( 1992; ). A simple method for estimating and testing minimum evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  12. Rzhetsky A. , Nei M. . ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  13. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  16. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed]
    [Google Scholar]
  17. Van Beilen J. B. , Li Z. , Duetz W. A. , Smits T. H. M. , Witholt B. . ( 2003; ). Diversity of alkane hydroxylase systems in the environment. . Oil Gas Sci Technol 58:, 427–440. [CrossRef]
    [Google Scholar]
  18. Venkateswaran K. , Moser D. P. , Dollhopf M. E. , Lies D. P. , Saffarini D. A. , MacGregor B. J. , Ringelberg D. B. , White D. C. , Nishijima M. et al. ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov.. Int J Syst Bacteriol 49:, 705–724. [CrossRef].[PubMed]
    [Google Scholar]
  19. Wang B. , Lai Q. , Cui Z. , Tan T. , Shao Z. . ( 2008; ). A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1. . Environ Microbiol 10:, 1948–1963. [CrossRef].[PubMed]
    [Google Scholar]
  20. Wang B. , Tan T. , Shao Z. . ( 2009; ). Roseovarius pacificus sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 59:, 1116–1121. [CrossRef].[PubMed]
    [Google Scholar]
  21. Wang L. , Wang W. , Lai Q. , Shao Z. . ( 2010; ). Gene diversity of CYP153A and AlkB alkane hydroxylases in oil-degrading bacteria isolated from the Atlantic Ocean. . Environ Microbiol 12:, 1230–1242. [CrossRef].[PubMed]
    [Google Scholar]
  22. Wu Y. , Lai Q. , Zhou Z. , Qiao N. , Liu C. , Shao Z. . ( 2009; ). Alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of Malacca and Singapore, producing a lipopeptide as its biosurfactant. . Int J Syst Evol Microbiol 59:, 1474–1479. [CrossRef].[PubMed]
    [Google Scholar]
  23. Yakimov M. M. , Golyshin P. N. , Lang S. , Moore E. R. B. , Abraham W. R. , Lünsdorf H. , Timmis K. N. . ( 1998; ). Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. . Int J Syst Bacteriol 48:, 339–348.[PubMed] [CrossRef]
    [Google Scholar]
  24. Yakimov M. M. , Timmis K. N. , Golyshin P. N. . ( 2007; ). Obligate oil-degrading marine bacteria. . Curr Opin Biotechnol 18:, 257–266. [CrossRef].[PubMed]
    [Google Scholar]
  25. Yamamoto S. , Kasai H. , Arnold D. L. , Jackson R. W. , Vivian A. , Harayama S. . ( 2000; ). Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. . Microbiology 146:, 2385–2394.[PubMed]
    [Google Scholar]
  26. Yuan J. , Lai Q. , Wang B. , Sun F. , Liu X. , Du Y. , Li G. , Gu L. , Zheng T. , Shao Z. . ( 2009; ). Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. . Int J Syst Evol Microbiol 59:, 1158–1161. [CrossRef].[PubMed]
    [Google Scholar]
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Transmission electron micrograph of negatively stained cells of strain W11-5 grown on 216L agar for 48 h at 28° C. Supplementary Figures [PDF](554kb)

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Cellular fatty acid contents of strain W11-5 and recognized species of the genus .  Supplementary Tables [PDF](51kb)

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