1887

Abstract

Among a large collection of South African dairy isolates, a novel taxon (DNA group 3) was previously delineated by a polyphasic taxonomic study ( Hugo ., , 586–595, 1999 ). In the present paper, this taxon is further characterized using 16S rDNA sequencing, fatty acid methyl ester analysis and a comparative phenotypic analysis, resulting in the proposal of a novel species, sp. nov. (type strain Ix 5a=LMG 18212=CCUG 46665).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02232-0
2003-05-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/3/ijs530771.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02232-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  2. Brown, V. I. & Lowbury, E. J. L. ( 1965; ). Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa. J Clin Pathol 18, 752–756.[CrossRef]
    [Google Scholar]
  3. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  4. Cowan, S. T. ( 1974; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press.
  5. Fautz, E. & Reichenbach, H. ( 1980; ). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8, 87–91.[CrossRef]
    [Google Scholar]
  6. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Phillips, G. B. ( 1981; ). Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  7. Holmes, B. ( 1992; ). The genera Flavobacterium, Sphingobacterium, and Weeksella. In The Prokaryotes, 2nd edn, vol. 4, pp. 3620–3630. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. Berlin: Springer.
  8. Holmes, B., Lapage, S. P. & Malnick, H. ( 1975; ). Strains of Pseudomonas putrefaciens from clinical material. J Clin Pathol 28, 149–155.[CrossRef]
    [Google Scholar]
  9. Holmes, B., Owen, R. J. & McMeekin, T. A. ( 1984a; ). Genus Flavobacterium Harrison, Breed, Hammer and Huntoon 1923, 97AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 353–361. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Holmes, B., Owen, R. J., Steigerwalt, A. G. & Brenner, D. J. ( 1984b; ). Flavobacterium gleum, a new species found in human clinical specimens. Int J Syst Bacteriol 34, 21–25.[CrossRef]
    [Google Scholar]
  11. Hugo, C. J. & Jooste, P. J. ( 1997; ). Preliminary differentiation of food strains of Chryseobacterium and Empedobacter using multilocus enzyme electrophoresis. Food Microbiol 14, 133–142.[CrossRef]
    [Google Scholar]
  12. Hugo, C. J., Jooste, P. J., Segers, P., Vancanneyt, M. & Kersters, K. ( 1999; ). A polyphasic taxonomic study of Chryseobacterium strains isolated from dairy sources. Syst Appl Microbiol 22, 586–595.[CrossRef]
    [Google Scholar]
  13. Jooste, P. J. ( 1985; ). The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources. PhD thesis, University of the Orange Free State, South Africa.
  14. Jooste, P. J., Britz, T. J. & de Haast, J. ( 1985; ). A numerical taxonomic study of Flavobacterium-Cytophaga strains from dairy sources. J Appl Bacteriol 59, 311–323.[CrossRef]
    [Google Scholar]
  15. Jooste, P. J., Britz, T. J. & Lategan, P. M. ( 1986a; ). The prevalence and significance of Flavobacterium strains in commercial salted butter. Milchwissenschaft 41, 69–73.
    [Google Scholar]
  16. Jooste, P. J., Britz, T. J. & Lategan, P. M. ( 1986b; ). Screening for the presence of Flavobacterium strains in dairy sources. S Afr J Dairy Sci 18, 45–50.
    [Google Scholar]
  17. King, E. O. ( 1959; ). Studies on a group of previously unclassified bacteria associated with meningitis in infants. Am J Clin Pathol 31, 241–247.
    [Google Scholar]
  18. Lijnen, H. R., Van Hoef, B., Ugwu, F., Collen, D. & Roelants, I. ( 2000; ). Specific proteolysis of human plasminogen by a 24 kDa endopeptidase from a novel Chryseobacterium sp. Biochemistry 39, 479–488.[CrossRef]
    [Google Scholar]
  19. MacFaddin, J. F. ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  20. Mudarris, M., Austin, B., Segers, P., Vancanneyt, M., Hoste, B. & Bernardet, J. F. ( 1994; ). Flavobacterium scophthalmum sp. nov., a pathogen of turbot (Scophthalmus maximus L.). Int J Syst Bacteriol 44, 447–453.[CrossRef]
    [Google Scholar]
  21. NCCLS ( 1983; ). Performance standards for antimicrobial disk susceptibility tests. Tentative standard, vol. 3, no. 14. Villanove, PA: National Committee for Clinical Laboratory Standards.
  22. Owen, R. J. & Snell, J. J. S. ( 1976; ). Deoxyribonucleic acid reassociation in the classification of flavobacteria. J Gen Microbiol 93, 89–102.[CrossRef]
    [Google Scholar]
  23. Reynolds, E. S. ( 1963; ). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17, 208–221.[CrossRef]
    [Google Scholar]
  24. Segers, P., Mannheim, W., Vancanneyt, M., de Brandt, K., Hinz, K.-H., Kersters, K. & Vandamme, P. ( 1993; ). Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. Int J Syst Bacteriol 43, 768–776.[CrossRef]
    [Google Scholar]
  25. Spurr, A. R. ( 1969; ). A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26, 31–43.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species identification in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Steyn, P. L., Segers, P., Vancanneyt, M., Sandra, P., Kersters, K. & Joubert, J. J. ( 1998; ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48, 165–177.[CrossRef]
    [Google Scholar]
  28. Takeuchi, M. & Yokota, A. ( 1992; ). Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium, and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum. J Gen Appl Microbiol 38, 465–482.[CrossRef]
    [Google Scholar]
  29. Ursing, J. & Bruun, B. ( 1991; ). Genotypic heterogeneity of Flavobacterium group IIb and Flavobacterium breve demonstrated by DNA-DNA hybridization. APMIS 99, 780–786.[CrossRef]
    [Google Scholar]
  30. Vancanneyt, M., Segers, P., Torck, U., Hoste, B., Bernardet, J.-F., Vandamme, P. & Kersters, K. ( 1996; ). Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides, as Myroides odoratus comb. nov. and Myroides odoratimimus sp. nov. Int J Syst Bacteriol 46, 926–932.[CrossRef]
    [Google Scholar]
  31. Vandamme, P., Vancanneyt, M., Pot, B. & 10 other authors ( 1992; ). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef]
    [Google Scholar]
  32. Vandamme, P., Bernardet, J.-F., Segers, P., Kersters, K. & Holmes, B. ( 1994; ). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef]
    [Google Scholar]
  33. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438.
    [Google Scholar]
  34. Venter, H. ( 1997; ). Purification and characterisation of a heat stable metalloprotease from a Chryseobacterium of dairy origin. MSc thesis, University of the Orange Free State, South Africa.
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Welthagen, J. J. & Jooste, P. J. ( 1992; ). Isolasie en karakterisering van gepigmenteerde psigrotrofe bakterieë uit verkoelde roumelk. S Afr J Dairy Sci 24, 47–52 (in Afrikaans).
    [Google Scholar]
  37. Yabuuchi, E., Kaneko, T., Yano, I., Moss, C. W. & Miyoshi, N. ( 1983; ). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33, 580–598.[CrossRef]
    [Google Scholar]
  38. Yamaguchi, S. & Yokoe, M. ( 2000; ). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66, 3337–3343.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02232-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02232-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 771 - 777

Susceptibilities of species and related bacteria to 26 antimicrobial agents. [PDF](15 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error