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Abstract

Two strains of Gram-reaction-negative, rod-shaped, non-spore-forming, non-motile, aerobic bacteria, designated LW30 and LW29, were isolated from the rhizosphere of a wetland reed in Dongtan, Chongming Island, China. The strains formed pale-yellow colonies on R2A plates. Growth occurred at 4–37 °C (optimum 30 °C), at pH 6–9 (optimum pH 7–8) and in the presence of 0–3 % (w/v) NaCl (optimum 0–1 %). Oxidase and catalase activities and flexirubin-type pigments were absent. MK-6 was the major respiratory quinone. The major fatty acids were iso-C, C, iso-C G and iso-C 9. Strains LW30 and LW29 could be differentiated from related species by several phenotypic characteristics. Phylogenetic analyses based on 16S rRNA gene sequences placed strains LW30 and LW29 in the genus with high sequence similarity to NJ-26 (94.0 %) and GPTSA 100-9 (93.9 %). Together with GPTSA 100-9, strains LW30 and LW29 formed a distinct group in the phylogenetic tree. The DNA G+C content was 30 mol%. On the basis of the phylogenetic and phenotypic evidence, strains LW30 and LW29 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LW30 (=KCTC 22671 =CCTCC AB 209201).

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2011-02-01
2019-10-22
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vol. , part 2, pp. 343 - 346

Fatty acid compositions (%) of strains LW30 and LW29 and related species of the genus Flavobacterium. [PDF](46KB)



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