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Abstract

A Gram-stain-positive, non-spore-forming actinobacterium, designated 15-Je-017, was isolated from wall material of an indoor environment. The isolate formed a rudimentary substrate mycelium that fragmented into rod-shaped cells. On the basis of 16S rRNA gene sequence analysis, strain 15-Je-017 was shown to belong to the genus and was most closely related to YIM 61503 (99.7 % 16S rRNA gene sequence similarity), D8-87 (99.0 %) and YIM 002 (99.0 %). The predominant menaquinone was MK-9(H). Whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid in the cell wall and rhamnose and glucose as the main sugars. Mycolic acids were absent. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and seven unknown phospholipids. The fatty acid profile contained major amounts (>5 %) of anteiso-C, iso-C, iso-C, iso-C, anteiso-C and C 8, which supported the affiliation of strain 15-Je-017 to the genus . DNA–DNA relatedness and physiological and biochemical tests allowed the differentiation of strain 15-Je-017 from the type strains of the three known species. Strain 15-Je-017 represents a novel species, for which we propose the name sp. nov., with type strain 15-Je-017 (=DSM 45357 =CCM 7680).

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2011-01-01
2019-10-20
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Phase-contrast micrographs of cells of sp. nov. 15-Je-017 after growth for 5 days in liquid M79 (a), 48 h on NA (b) and 72 h on NA (c).

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Micrographs of colonies of sp. nov. 15-Je-017 after growth for 10 days on NA at 28 °C using stereomicroscopy at ×10 magnification (a) and phase-contrast microscopy at ×400 magnification (b).

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