1887

Abstract

Three aniline-degrading bacteria, strains DN316, DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus , with ( = ) LMG 140 as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316 using sequences of the , and genes and the 16S–23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA–DNA relatedness between DN316 and LMG 140 was 43.7 %, clearly indicating that the representative strain DN316 represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were Cω7 (57.10 %), C (11.31 %) and C cyclo ω8 (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DN316 ( = CICC 10378  = LMG 23925).

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2011-04-01
2019-12-05
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vol. , part 4, pp. 816 - 822

Neighbour-joining trees based on partial sequences of the (Fig. S1), (Fig. S2) and (Fig. S3) genes and the 16S–23S intergenic spacer (Fig. S4) of species of the genus and other related organisms. [PDF](49 KB)



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