1887

Abstract

A Gram-positive, aerobic, non-motile actinobacterium, designated strain 6014, was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014 contained arabinose, galactose and ribose as diagnostic sugars and -diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, -acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H) and MK-9(H). The major fatty acids were C, i-C, C and 10-methyl-C. The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014 fell within the radius of the suborder , in which the strain formed a distinct lineage next to genera of the family Based on data from this polyphasic study, strain 6014 can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder . The name gen. nov., sp. nov. is proposed with 6014 (=DSM 45181 =KCTC 19655) as the type strain.

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2011-03-01
2019-10-14
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vol. , part 3, pp. 592 - 597

Polar lipid profile of strain 6014 grown in ISP 4 medium. PL, unknown phospholipids; PME, phosphatidylmethylethanolamine; PE, phosphatidylethanolamine; OH-PE, hydroxy-phosphatidylethanolamine; GluNu, -acetylglucosamine-containing phospholipids; GL, unknown glycolipids.

71 carbon source utilization assays in Biolog GEN III (MicroPlate ) of strain 6014

Dextrin

+

l-Rhamnose

+

d-Glucuronic acid

+

Maltose

+

Inosine

+

Glucuronamide

+

Trehalose

+

d-Sorbitol

+

Mucic acid

+

Cellobiose

+

d-Mannitol

+

Quinic acid

+

Gentiobiose

+

d-Arabitol

+

d-Saccharic acid

+

Sucrose

+

-Inositol

p-Hydroxyphenylacetic acid

+

Turanose

+

Glycerol

+

Methyl pyruvate

+

Stachyose

+

d-Glucose 6-phosphate

+

d-Lactic acid methyl ester

+

Raffinose

+

d-Fructose 6-phosphate

+

l-Lactic acid

+

α-Lactose

d-Aspartic acid

+

Citric acid

+

Melibiose

+

d-Serine

+

α-Keto-glutaric acid

+

β-Methyl- d-glucoside

+

Gelatin

+

d-Malic acid

+

d-Salicin

+

Glycyl l-proline

+

l-Malic acid

+

-Acetyl- d-glucosamine

+

l-Alanine

+

Bromosuccinic acid

+

-Acetyl-β- d-mannosamine

+

l-Arginine

+

Tween 40

+

-Acetyl- d-galactosamine

+

l-Aspartic acid

+

γ-Aminobutryric acid

+

-Acetyl neuraminic acid

+

l-Glutamic acid

+

α-Hydroxybutyric acid

+

α- d-Glucose

+

l-Histidine

+

β-Hydroxy- dl-butyric acid

+

d-Mannose

+

l-Pyroglutamic acid

+

α-Ketobutyric acid

+

d-Fructose

+

l-Serine

+

Acetoacetic acid

+

d-Galactose

Pectin

+

Propionic acid

+

3-Methyl glucose

+

d-Galacturonic acid

+

Acetic acid

+

d-Fucose

+

l-Galactonic acid lactone

+

Formic acid

+

l-Fucose

+

d-Gluconic acid

+

 

 



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