1887

Abstract

A Gram-positive, non-motile, non-spore-forming, aerobic, coccoid-shaped bacterium, designated BXN5-13, was isolated from the soil of a ginseng field from Baekdu Mountain in Jilin district, China. Strain BXN5-13 grew optimally at 30 °C and pH 6.5–7.5 with 0–2 % (w/v) NaCl. Strain BXN5-13 had -glucosidase activity that was connected with ginsenoside-converting ability, so that it was able to convert ginsenoside Rb to ginsenoside F2. On the basis of 16S rRNA gene sequence analysis, the closest phylogenetic relatives of strain BXN5-13 were 5516T-20 (98.4 % 16S rRNA gene sequence similarity), MSL-03 (98.3 %), DS-8 (97.9 %) and KSW2-15 (96.9 %). Lower sequence similarity (<97.0 %) was found with the type strains of other recognized species of the family . The predominant quinone was MK-8(H). The major fatty acids (>10 %) were iso-C, C, anteiso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. The cell-wall peptidoglycan contained -diaminopimelic acid. The chemotaxonomic data and the high genomic DNA G+C content of strain BXN5-13 (70.8 mol%) supported its affiliation with the genus . DNA–DNA relatedness between strain BXN5-13 and its closest phylogenetic neighbours was below 16 %. Strain BXN5-13 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BXN5-13 (=KCTC 19419=DSM 21006=LMG 24462).

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2011-03-01
2024-03-28
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References

  1. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  2. Choi K.-T. 2008; Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin 29:1109–1118 [CrossRef]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  4. Dastager S. G., Lee J.-C., Ju Y.-J., Park D.-J., Kim C.-J. 2008; Phycicoccus bigeumensis sp. nov., a mesophilic actinobacterium isolated from Bigeum Island, Korea. Int J Syst Evol Microbiol 58:2425–2428 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [CrossRef]
    [Google Scholar]
  10. Kim M.-K., Lee J.-W., Lee K.-Y., Yang D.-C. 2005; Microbial conversion of major ginsenoside Rb1 to pharmaceutically active minor ginsenoside Rd. J Microbiol 43:456–462
    [Google Scholar]
  11. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  13. Kumar S., Dudley J., Nei M., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [CrossRef]
    [Google Scholar]
  14. Lee S. D. 2006; Phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed. Int J Syst Evol Microbiol 56:2369–2373 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  17. Moore D. D., Dowhan D. 1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp 2–11 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  18. Park J. H. 2004; Sun ginseng – a new processed ginseng with fortified activity. Food Ind Nutr 9:23–27
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  21. Son J.-W., Kim H.-J., Oh D.-K. 2008; Ginsenoside Rd production from the major ginsenoside Rb1 by β -glucosidase from Thermus caldophilus . Biotechnol Lett 30:713–716 [CrossRef]
    [Google Scholar]
  22. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  23. Ten L. N., Jung H.-M., Yoo S.-A., Im W.-T., Lee S.-T. 2008; Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea. J Microbiol 46:519–524 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  26. Weon H.-Y., Yoo S.-H., Kim B.-Y., Schumann P., Kroppenstedt R. M., Hong S.-K., Kwon S.-W. 2008; Phycicoccus aerophilus sp. nov., isolated from air. Int J Syst Evol Microbiol 58:2389–2392 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Lee S.-Y., Kang S.-J., Oh T.-K. 2008; Phycicoccus dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 58:597–600 [CrossRef]
    [Google Scholar]
  28. Zhao X., Wang J., Li J., Fu L., Gao J., Du X., Bi H., Zhou Y., Tai G. 2009; Highly selective biotransformation of ginsenoside Rb1 to Rd by the phytopathogenic fungus Cladosporium fulvum (syn. Fulvia fulva ). J Ind Microbiol Biotechnol 36:721–726 [CrossRef]
    [Google Scholar]
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