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Abstract

A Gram-positive, non-motile, non-spore-forming, aerobic, coccoid-shaped bacterium, designated BXN5-13, was isolated from the soil of a ginseng field from Baekdu Mountain in Jilin district, China. Strain BXN5-13 grew optimally at 30 °C and pH 6.5–7.5 with 0–2 % (w/v) NaCl. Strain BXN5-13 had -glucosidase activity that was connected with ginsenoside-converting ability, so that it was able to convert ginsenoside Rb to ginsenoside F2. On the basis of 16S rRNA gene sequence analysis, the closest phylogenetic relatives of strain BXN5-13 were 5516T-20 (98.4 % 16S rRNA gene sequence similarity), MSL-03 (98.3 %), DS-8 (97.9 %) and KSW2-15 (96.9 %). Lower sequence similarity (<97.0 %) was found with the type strains of other recognized species of the family . The predominant quinone was MK-8(H). The major fatty acids (>10 %) were iso-C, C, anteiso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. The cell-wall peptidoglycan contained -diaminopimelic acid. The chemotaxonomic data and the high genomic DNA G+C content of strain BXN5-13 (70.8 mol%) supported its affiliation with the genus . DNA–DNA relatedness between strain BXN5-13 and its closest phylogenetic neighbours was below 16 %. Strain BXN5-13 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BXN5-13 (=KCTC 19419=DSM 21006=LMG 24462).

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2011-03-01
2019-10-23
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Two-dimensional TLC of polar lipids of sp. nov. BXN5-13 . Chloroform/methanol/water (65:25:4) was used in the first direction, followed by chloroform/acetic acid/methanol/water (80:15:12:4) in the second direction. The spray reagent 5% ethanolic molybdatophosphoric acid was used to detect total lipids. AL, Unknown aminolipid; DPG, diphosphatidylglycerol; L, unknown lipid; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol.

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Cellular fatty acid profiles of sp. nov. BXN5-13 and the type strains of species of the genus . [ PDF] 41 KB

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